SubtiBank SubtiBank
tcyB
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tcyB

cystine and diaminopimelate [SW|ABC transporter] (permease)
locus
BSU_03600
pI
9.93
mw
26.24 kDa
protein length
234 aa Sequence Blast
gene length
705 bp Sequence Blast
function
cystine and diaminopimelate uptake
product
cystine and diaminopimelate [SW|ABC transporter] (permease), membrane protein
essential
no
synonyms
yckJ

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.2|Uptake of amino acids]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.9|Biosynthesis/ acquisition of cysteine]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    409,965 410,669

    The protein

    Catalyzed reaction/ biological activity

  • uptake of cystine and diaminopimelate [pubmed|29995990]
  • Protein family

  • [SW|Binding-protein-dependent transport system permease family] (according to UniProt)
  • Paralogous protein(s)

  • [protein|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|YxeN], [protein|1872614A6F7DBF7231348AC3DAE4ED3295ACEB41|YckA]
  • Structure

  • [PDB|4YMS] (amino acid transporter from Caldanaerobacter subterraneus, 37% identity) [pubmed|25848002]
  • [SW|Localization]

  • membrane protein [Pubmed|10092453,18763711]
  • Expression and Regulation

    Operons

    genes
    [gene|2056F8EEDF9D3027CFD808E8D0C5DF544DBDD3F3|tcyA]-[gene|9400DF25487461E3738DDDC72BDDB1589281409D|tcyB]-[gene|B453986691E1231A1C565D469A2A60EBFF2F6BD3|tcyC]
    description
    [Pubmed|22383849,15262924]

    regulation

  • strongly repressed in response to glucose starvation in M9 medium [Pubmed|23033921]
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    Biological materials

    Mutant

  • MGNA-C059 (yckJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2057 NBRP B. subtilis, Japan]
  • BKE03600 ([gene|9400DF25487461E3738DDDC72BDDB1589281409D|tcyB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTGCCAAGCGTCAATGCCG, downstream forward: _UP4_GTGGCCAAATAAGGAGGTTC
  • BKK03600 ([gene|9400DF25487461E3738DDDC72BDDB1589281409D|tcyB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTGCCAAGCGTCAATGCCG, downstream forward: _UP4_GTGGCCAAATAAGGAGGTTC
  • References

  • 15262924,10092453,7551042,18763711,25848002,29995990