SubtiBank SubtiBank
csrA [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]
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csrA [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

motility regulator, binds to the hag mRNA to inhibit its translation
locus
BSU35370
pI
6.00
mw
8.00 kDa
function
control of hag translation
product
motility regulator
essential
no
synonyms
yviG

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.4|RNA binding regulators]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.5|Additional chemotaxis signal transduction and regulatory proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|motility and chemotaxis]
  • This gene is a member of the following [SW|regulons]

  • [SW|SigD regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    3,636,046 -> 3,636,270

    The protein

    Catalyzed reaction/ biological activity

  • [protein|search|CsrA] binds the ''[protein|search|hag]'' mRNA to inhibit translation [Pubmed|21895793]
  • Protein family

  • csrA family (according to Swiss-Prot)
  • Effectors of protein activity

  • [protein|search|CsrA] is sequestered by interaction with [protein|search|FliW] (the two proteins form a conserved module in many bacteria) [Pubmed|21895793,27516547]
  • Structure

  • [PDB|1T3O]
  • [PDB|5DMB] ([SW|FliW]-[SW|CsrA] complex) [Pubmed|27551070]
  • [SW|Interactions]

  • [protein|search|FliW]-[protein|search|CsrA] [Pubmed|21895793,27516547]
  • Additional information

  • [protein|search|CsrA] and [protein|search|FliW] form a conserved module in many bacteria [Pubmed|21895793]
  • Expression and Regulation

    Operon

  • ''csrA'' [Pubmed|8969505]
  • [SW|Sigma factor]

  • [SW|SigD] [Pubmed|26577401]
  • Biological materials

    Mutant

  • GP469 (spc), available in [SW|Stülke] lab
  • Expression vector

  • pGP381 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab
  • for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP383, available in [SW|Stülke] lab
  • lacZ fusion

  • pGP461 (in [protein|search|pAC7]), available in [SW|Stülke] lab
  • References

    Reviews

  • 19385727,22672726,25251856
  • Original Publications

  • 16822857,17555441,8969505,21895793,23144244,26577401,27516547,27551070