SubtiBank SubtiBank
yhcA
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yhcA

c-di-AMP exporter
locus
BSU_09010
pI
9.49
mw
58.17 kDa
protein length
532 aa Sequence Blast
gene length
1599 bp Sequence Blast
function
secretion of cyclic di-AMP
product
c-di-AMP exporter
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.11|Other exporters]
  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    977,775 979,373

    The protein

    Catalyzed reaction/ biological activity

  • secretion of cyclic di-AMP [pubmed|29588402]
  • Protein family

  • [SW|major facilitator superfamily] (according to UniProt)
  • [SW|EmrB family] (according to UniProt)
  • Paralogous protein(s)

  • [protein|188742B9A0C95344D32A4C23B9C971660572CF7D|YcnB], [protein|23483430AE0381406EF2349A18CFAE1F14F9B180|LmrB]
  • Structure

  • [PDB|6E8J] (from Hyphomonas neptunium, corresponds to aa 93 ... 204, 28% identity)
  • [SW|Localization]

  • cell membrane (according to UniProt)
  • Expression and Regulation

    Operons

    genes
    [gene|EDDD61CF725449E57C72F63D378F6CD1FAC49ADA|yhbI]-[gene|211C58A92BA2A06A34B1F73BDB68A0F581B942C2|yhbJ]-[gene|93159DC7D5C42CFA54F66F232D73F38957243AB7|yhcA]-[gene|7797B0B66922D51B9D142DD2E5857836E47F015B|yhcB]-[gene|95EED3A91483502CA78067421F678B1EDB965B18|yhcC]
    description
    [Pubmed|22383849]

    additional information

  • the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] (the half-life of the mRNA increases from 4 to 41 min) [PubMed|21815947]
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    Biological materials

    Mutant

  • MGNA-B471 (yhcA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1470 NBRP B. subtilis, Japan]
  • GP1704 (''[gene|93159DC7D5C42CFA54F66F232D73F38957243AB7|yhcA]''::''ermC'', without terminator), available in [SW|Jörg Stülke]'s lab
  • BKE09010 ([gene|93159DC7D5C42CFA54F66F232D73F38957243AB7|yhcA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAGACAACCTCCTTT, downstream forward: _UP4_TAATCTGTACAGGGAGGTTT
  • BKK09010 ([gene|93159DC7D5C42CFA54F66F232D73F38957243AB7|yhcA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAGACAACCTCCTTT, downstream forward: _UP4_TAATCTGTACAGGGAGGTTT
  • References

  • 21815947,29588402