SubtiBank SubtiBank
cdaS [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]
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cdaS [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

sporulation-specific diadenylate cyclase, synthesis of c-di-AMP
locus
BSU19430
pI
5.00
mw
22.00 kDa
function
synthesis of c-di-AMP
product
sporulation-specific diadenylate cyclase
essential
no
ec
2.7.7.85
synonyms
yojJ

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [SW|Categories] containing this gene/protein

  • [SW|metabolism of signalling nucleotides], [SW|sporulation proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|SigG regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,118,504 -> 2,119,127

    The protein

    Catalyzed reaction/ biological activity

  • synthesis of c-di-AMP from two molecules of ATP [Pubmed|23192352]
  • Paralogous protein(s)

  • [protein|search|CdaA]
  • [SW|Domains]

  • N-terminal autoinhibitory domain [Pubmed|24939848]
  • C-terminal [SW|DAC domain] involved in the synthesis of c-di-AMP [Pubmed|21566650]
  • Structure

  • [PDB|2FB5] (the protein of ''B. cereus'', 48% identity, 81% similarity)
  • [SW|Interactions]

  • forms hexamers (enzymatically inactive) or dimers (active) [Pubmed|24939848]
  • Expression and Regulation

    Operon

  • ''[protein|search|cdaS]'' [Pubmed|22383849]
  • [SW|Sigma factor]

  • [protein|search|SigG] [Pubmed|22383849]
  • Regulation

  • expressed late during sporulation in the forespore ([protein|search|SigG]) [Pubmed|22383849]
  • Biological materials

    Mutant

  • GP983 (''[protein|search|cdaS]''::''ermC''), available in [SW|Jörg Stülke]'s lab
  • GP1360 (''[protein|search|cdaS]''::''spc''), available in [SW|Jörg Stülke]'s lab
  • Expression vector

  • IPTG inducible expression of '''''B. cereus''''' Strep-''cdaS'' in ''E. coli'': pGP2593 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • References

    Reviews

  • 25869574,18714086,23812326
  • Original publications

  • 21566650,23192352,22383849,24939848,26441857