SubtiBank SubtiBank
ktrA [Sun Jan 15 2017 12:32:53 GMT+0100 (CET)]
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ktrA [Sun Jan 15 2017 12:32:53 GMT+0100 (CET)]

high affinity potassium transporter KtrA-KtrB, peripheric membrane component (proton symport)
24.00 kDa
potassium uptake
high affinity potassium transporter KtrA-KtrB,
peripheric membrane component (proton symport)

Genomic Context



  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW|Potassium uptake/ export]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|transporters/ other], [SW|metal ion homeostasis (K, Na, Ca, Mg)], [SW|coping with hyper-osmotic stress], [SW|membrane proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|YdaO riboswitch]
  • Gene

    Coordinates on the chromosome (coding sequence)
    3,188,414 -> 3,189,082

    Phenotypes of a mutant

  • The ''[protein|search|ktrA]-[protein|search|ktrB]'' mutant of ''B. subtilis'' NCIB3610 is reduced in sliding (dendritic spreading) [Pubmed|16321950]
  • The protein

    Paralogous protein(s)

  • [protein|search|KtrC]
  • [SW|Domains]

  • contains a [SW|RCK_N domain] at the N-terminus (according to [ N-terminal domain* UniProt])
  • contains a [SW|RCK_C domain] at the C-terminus (according to [ C-terminal domain* UniProt])
  • Structure

  • [PDB|4J7C] (the [protein|search|KtrA]-[protein|search|KtrB] complex) [Pubmed|23598340]
  • [PDB|4XTT] (the ''S. aureus'' KtrA [SW|RCK_C domain] in complex with c-di-AMP, 48% identity) [Pubmed|25957408]
  • [PDB|1LSU] (complex with NADH)
  • [ 2HMW] ( complex with ATP)
  • [SW|Localization]

  • peripheral membrane protein [Pubmed|12562800]
  • [SW|Interactions]

  • [protein|search|KtrA]-[protein|search|KtrB] [Pubmed|12562800], composed of an octameric [protein|search|KtrA] ring and two [protein|search|KtrB] dimers [Pubmed|23598340]
  • Expression and Regulation


  • ''[protein|search|ktrA]-[protein|search|ktrB]'' [Pubmed|12562800]
  • Regulatory mechanism

  • expression is controlled via termination antitermination by the [SW|ydaO riboswitch], expression is switched off upon binding of c-di-AMP [Pubmed|24141192,20511502]
  • Biological materials


  • MGNA-B543 (yuaA::erm), available at the [ NBRP B. subtilis, Japan]
  • 1A954 ( ''ktrA''::''kan''), [Pubmed|12562800], available at [ BGSC]
  • GHB1 (D(''[protein|search|ktrA]-[protein|search|ktrB]'')::''aphA3''), available in [SW|Erhard Bremer]'s lab
  • GP92 (D(''[protein|search|ktrA]-[protein|search|ktrB]'')::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • GP2083 (D(''[protein|search|ktrA]-[protein|search|ktrB]'')::''aphA3'' D''[protein|search|ktrC]''::''tet''), available in [SW|Jörg Stülke]'s lab
  • Expression vector

  • pGP2594: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • lacZ fusion

  • GP2176 (based on [SW|pAC5]), available in [SW|Jörg Stülke]'s lab
  • GP2177 (based on [SW|pAC7]), available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Erhard Bremer], University of Marburg, Germany [ homepage]
  • References


  • 25869574,27935846,28086088
  • Original publications

  • 12562800,15096624,16321950,20511502,23086297,23598340,16990138,24141192,25957408,16990138,26771197,28086091