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ktrA [2019-02-14 12:07:16]

high affinity potassium channel [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]-[protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB], peripheric membrane component

locus: BSU31090

pI: 5.98

mw: 24.73 kDa

protein length: 222 aa

gene length: 669 bp

function: potassium uptake

product: high affinity potassium channel [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]-[protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB], peripheric membrane component

essential: no

synonyms: yuaA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,188,414 3,189,082

Phenotypes of a mutant

• a [gene|search|kimA ][gene|search|ktrA ][gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB] mutant requires high potassium concentrations on minimal medium with ammonium as nitrogen source [pubmed|28420751,28679749]
• a [gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB] mutant of ''B. subtilis'' NCIB3610 is reduced in sliding (dendritic spreading) [Pubmed|16321950]

The protein

Paralogous protein(s)

• [protein|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|KtrC]

[SW|Domains]

• contains a [SW|RCK_N domain] at the N-terminus (according to [http://www.uniprot.org/uniprot/?query=RCK+N-terminal+domain&sort=score UniProt])
• contains a [SW|RCK_C domain] at the C-terminus (according to [http://www.uniprot.org/uniprot/?query=RCK+C-terminal+domain&sort=score UniProt])

Effectors of protein activity

• binds ADP and ATP [pubmed|26771197]

Structure

• [PDB|4J7C] (the [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]-[protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB] complex) [Pubmed|23598340]
• [PDB|4XTT] (the ''S. aureus'' KtrA [SW|RCK_C domain] in complex with c-di-AMP, 48% identity) [Pubmed|25957408]
• [PDB|1LSU] (complex with NADH)
• [http://www.proteopedia.org/wiki/index.php/2hmw 2HMW] ( complex with ATP)

[SW|Localization]

• peripheral membrane protein [Pubmed|12562800]

Expression and Regulation

Operons

• genes: [gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]
  description: [Pubmed|12562800]

  regulatory mechanism

  • [regulon|ydaO riboswitch|ydaO riboswitch]: termination/antitermination, expression is switched off upon binding of c-di-AMP, in [regulon|ydaO riboswitch|ydaO riboswitch]

additional information

• growth at extreme potassium limitation results in the acquisition of promoter mutations with increased [gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB] expression [pubmed|28679749]

Biological materials

Mutant

• MGNA-B543 (yuaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1542 NBRP B. subtilis, Japan]
• 1A954 ( ''ktrA''::''kan''), [Pubmed|12562800], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A954&Search=1A954 BGSC]
• GHB1 ([gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::''aphA3''), available in [SW|Erhard Bremer]'s lab
• GP92 ([gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::''aphA3''), available in [SW|Jrg Stlke]'s lab [pubmed|28420751]
• GP2083 ([gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::''aphA3'' [gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]::''tet''), available in [SW|Jrg Stlke]'s lab [pubmed|28420751]
• GP2498 ([gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::''spc'' [gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]::''cat''), available in [SW|Jrg Stlke]'s lab
• BKE31090 ([gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTTCATATCTCCCTTA, downstream forward: _UP4_GAAAACGAAGGGATGTAGAC
• BKK31090 ([gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTTCATATCTCCCTTA, downstream forward: _UP4_GAAAACGAAGGGATGTAGAC
• GP2716 ([gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::''spc''), available in [SW|Jrg Stlke]'s lab

Expression vectors

• pGP2594: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jrg Stlke]'s lab

lacZ fusion

• GP2176 (based on [SW|pAC5]), available in [SW|Jrg Stlke]'s lab
• GP2177 (based on [SW|pAC7]), available in [SW|Jrg Stlke]'s lab
• GP2299 (based on [SW|pAC6]), available in [SW|Jrg Stlke]'s lab [pubmed|28679749]

References

Reviews

• 25869574,27935846,28086088,28825218,25838295

Original publications

• 12562800,15096624,16321950,20511502,23086297,23598340,24141192,25957408,16990138,26771197,28086091,28420751,28504641,28679749

Labs working on this gene/protein

• [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]