slrR [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

transcriptional activator (Xre family) of competence development and sporulation genes, represses SigD-dependent flagellar genes, antagonist of SlrA and SinR, has LexA-like autocleavage activity

locus: BSU34380

pI: 9.00

mw: 17.00 kDa

function: regulation of initiation of biofilm formation and of autolysis

product: transcriptional regulator (Xre family), SlrA antagonist

essential: no

synonyms: yveJ, slr

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.6|Transition state regulators]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.4|Swarming]

[SW|Categories] containing this gene/protein

• [SW|transcription factors and their control], [SW|transition state regulators], [SW|biofilm formation]

This gene is a member of the following [SW|regulons]

• [SW|Abh regulon], [SW|AbrB regulon], [SW|SinR regulon]

Gene

Coordinates on the chromosome (coding sequence): 3,530,101 -> 3,530,559

Phenotypes of a mutant

• smooth colonies on MsGG medium, no [SW|biofilm formation] [Pubmed|22113911]

The protein

Catalyzed reaction/ biological activity

• [protein|search|SlrR] binds to and inhibits the activity of [protein|search|SlrA], [protein|search|SlrA] indirectly stimulates the synthesis of [protein|search|SlrR] by interacting with [protein|search|SinR]. [protein|search|SlrR] can bind to [protein|search|SinR] and [protein|search|SinR] directly represses the transcription of [protein|search|SlrR]. [protein|search|SlrR] indirectly derepresses its own gene. The heterocomplex of [protein|search|SlrR]-[protein|search|SinR] is a repressor of autolysin and motility genes and inhibits the repressor function of [protein|search|SinR]. [Pubmed|19788541,20923420,20351052]
• repression of transcription of ''[protein|search|lytA]-[protein|search|lytB]-[protein|search|lytC]'' and ''[protein|search|lytF]'' [Pubmed|20351052]
• autocleavage [Pubmed|20923420]

Protein family

• [SW|Xre family]

Paralogous protein(s)

• [protein|search|SinR]

Modification

• subject to self-cleavage via a LexA-like autopeptidase, this involves [protein|search|ClpC] and requires [SW|DegU] [Pubmed|26819068,20923420]

Effectors of protein activity

• interaction with [protein|search|SinR] triggers binding of SlrR to the promoters of ''[protein|search|lytA]-[protein|search|lytB]-[protein|search|lytC]'' and ''[protein|search|lytF]'', resulting in their repression [Pubmed|20351052]

[SW|Interactions]

• [protein|search|SlrA]-[protein|search|SlrR] [Pubmed|19788541]
• [protein|search|SlrR]-[protein|search|SinR] [Pubmed|20351052], K(D) 47.5 nM [Pubmed|23430750], [protein|search|Veg] may inhibit this interaction [Pubmed|23378512]

Expression and Regulation

Operon

• ''[protein|search|slrR]'' [Pubmed|21815947]

Regulatory mechanism

• [protein|search|Abh]: transcription activation [Pubmed|19767430]
• [protein|search|SinR]: transcription repression [Pubmed|19788541]
• [protein|search|AbrB]: transcription repression [Pubmed|20817675]

Additional information

• induction by sequestration of [protein|search|SinR] by [protein|search|SinI], [protein|search|SlrA] [PubMed|15661000,19788541] or by SlrR itself [PubMed|20351052]
• the '[protein|search|slrR]' gene is not expressed in a '[protein|search|ymdB]' mutant [PubMed|21856853]
• the amount of the mRNA is substantially decreased upon depletion of [Rny|search|RNase Y] (this is likely due to the increased stability of the '[protein|search|sinR]' mRNA) [PubMed|21815947]

Biological materials

Mutant

• GP955 (''slrR''-''[protein|search|pnbA]''::''cat''), available in [SW|Jörg Stülke]'s lab

Expression vector

• pGP2905: expression in ''E. coli'', with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]' lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in [SW|Jörg Stülke]' lab

References

Reviews

• 20541494,23353768,24988880

Original publications

• 23378512,23430750,21856853,22329926,22113911,20817675,18430133,18647168,19767430,19788541,20351052,20923420,24256735,26819068