yncB

similar to micrococcal nuclease

locus: BSU_17620

pI: 9.21

mw: 23.54 kDa

protein length: 211 aa

gene length: 636 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 1,895,378 1,896,013

The protein

Protein family

• thermonuclease family (single member, according to UniProt)

Paralogous protein(s)

• [protein|27F32FB0F96276325ED93710DF77B949658D728D|YokF]

Structure

• [PDB|4I65] (from Staphylococcus aureus, corresponds to aa 69 ... 169 of YncB, 58% identity)

[SW|Localization]

• Cytoplasm (homogeneous) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|91E6B4971F22B5A5F964590F8B14D6C73A4C4840|yncB]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B100 (yncB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1099 NBRP B. subtilis, Japan]
• BKE17620 ([gene|91E6B4971F22B5A5F964590F8B14D6C73A4C4840|yncB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGATTCCCTCTTTAT, downstream forward: _UP4_TAGAAAAACACTGTTTGTTA
• BKK17620 ([gene|91E6B4971F22B5A5F964590F8B14D6C73A4C4840|yncB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGATTCCCTCTTTAT, downstream forward: _UP4_TAGAAAAACACTGTTTGTTA

References

• 16479537