yknW

modulates assembly of the [protein|FD270195901B89DAAAD134DB9DE729670A9BDECF|YknX]-[protein|FE3A01BEA974C5E32C68DC600C800E5EF101B34F|YknY]-[protein|9680326BAA94A503E2A5C6F0A2479AE0B0467B4C|YknZ] [SW|ABC transporter] for the export of the [protein|820635420B3980B620F3E3D7ACF7EDE9DC6275AD|SdpC] toxin

locus: BSU_14340

pI: 6.94

mw: 24.03 kDa

protein length: 231 aa

gene length: 696 bp

function: resistence against [protein|820635420B3980B620F3E3D7ACF7EDE9DC6275AD|SdpC] toxin

product: modulator of [SW|ABC transporter] assembly

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,503,582 1,504,277

The protein

[SW|Localization]

• cell membrane (according to UniProt), membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|70D7098BEBD5E53B4B9B8C3394FAAF972C65EE22|yknU]-[gene|C6B0ACB9D9703DD8141FD7D9D62DB22FB48F458B|yknV]-[gene|916419C4ED113778488EBC558A35810498028B02|yknW]-[gene|FD270195901B89DAAAD134DB9DE729670A9BDECF|yknX]-[gene|FE3A01BEA974C5E32C68DC600C800E5EF101B34F|yknY]-[gene|9680326BAA94A503E2A5C6F0A2479AE0B0467B4C|yknZ]
  description: [Pubmed|22383849]

• genes: [gene|916419C4ED113778488EBC558A35810498028B02|yknW]-[gene|FD270195901B89DAAAD134DB9DE729670A9BDECF|yknX]-[gene|FE3A01BEA974C5E32C68DC600C800E5EF101B34F|yknY]-[gene|9680326BAA94A503E2A5C6F0A2479AE0B0467B4C|yknZ]
  description: [Pubmed|9987136]

  sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|9987136], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|12076816], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • repressed during logrithmic growth ([protein|search|AbrB]) [Pubmed|12076816]

Biological materials

Mutant

• MGNA-B348 (yknW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1347 NBRP B. subtilis, Japan]
• BKE14340 ([gene|916419C4ED113778488EBC558A35810498028B02|yknW]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCAAAACCTCCTTGAG, downstream forward: _UP4_AATTCTGTGGCGGGTGCATA
• BKK14340 ([gene|916419C4ED113778488EBC558A35810498028B02|yknW]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCAAAACCTCCTTGAG, downstream forward: _UP4_AATTCTGTGGCGGGTGCATA

References

• 16629676,12076816,18763711,9987136