SubtiBank SubtiBank
scpB [2017-02-27 15:02:04]
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scpB [2017-02-27 15:02:04]

part of the condensin complex, chromosomal origin condensation and segregation
locus
BSU23210
pI
4.00
mw
21.00 kDa
protein length
197 aa Sequence Blast
gene length
591 bp Sequence Blast
function
segregation of replication origins
product
DNA segregation and condensation protein
essential
yes
synonyms
ypuH

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
  • This gene is a member of the following [SW|regulons]

  • [SW|stringent response]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,425,248 -> 2,425,841

    Phenotypes of a mutant

  • essential [Pubmed|24440399], non-essential according to [Pubmed|28189581]
  • ''[gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth [Pubmed|24440399,24440393]
  • The protein

    Protein family

  • scpB family (according to Swiss-Prot)
  • Structure

  • [PDB|4I98] (complex between [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|ScpA] (aa 1-160)-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|ScpB] (aa 1-183) [Pubmed|23353789]
  • [SW|Localization]

  • nucleoid (Multiple) [Pubmed|16479537]
  • the [SW|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|Smc])2-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|ScpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|ScpB] complex is loaded by [protein|EF4BD49FCD49EE97908973581478951C8FA196C0|ParB] to ''parS'' centromeric sites adjacent to the replication origin, travels then from the origin to the terminus at rates >50 kb per minute [Pubmed|28154080,24440393,23475963]
  • Expression and Regulation
    [[this]]

    Biological materials

    Mutant

  • MGNA-A105 (ypuH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/105 NBRP B. subtilis, Japan]
  • References

    Reviews

  • 22933559,22934648,24118085,25460803,26706151
  • Original publications

  • 12100548,12065423,12421306,22385855,16479537,19450516,12897137,7934830,11948165,23353789,24440399,24440393,25071173,25557547,25951515,26253537,23475963,26295962,26725510,26904953,28189581,28154080