ntdR

transcription activator of the [gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]-[gene|A4DE6C394B64813A732255734758403241FFAC2F|ntdB]-[gene|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|ntdC]-[gene|search|glcP ]operon

locus: BSU_10560

pI: 6.64

mw: 37.53 kDa

protein length: 329 aa

gene length: 990 bp

function: regulation of the [gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]-[gene|A4DE6C394B64813A732255734758403241FFAC2F|ntdB]-[gene|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|ntdC]-[gene|search|glcP ]operon

product: transcriptional regulator ([SW|LacI family])

essential: no

synonyms: yhjM

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

Gene

Coordinates: 1,129,715 1,130,704

The protein

Catalyzed reaction/ biological activity

• transcription activation of the ''[gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]-[gene|A4DE6C394B64813A732255734758403241FFAC2F|ntdB]-[gene|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|ntdC]-[gene|ADB476B0B385D9B9CAF02902CE115C8309F67911|glcP]'' operon [Pubmed|17056753]

Protein family

• [SW|LacI family]

[SW|Domains]

• [SW|HTH lacI-type domain] (aa 2-56) (according to UniProt)

Structure

• [PDB|2HSG] ([protein|search|ccpA ]from B. megaterium, 31% identity) [pubmed|17500051]

Expression and Regulation

Operons

• genes: [gene|9118A36CD30E7C99697B3659BB04E03BB7EE71D7|ntdR]
  description: [Pubmed|14612444]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

Biological materials

Mutant

• MGNA-A726 (yhjM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/726 NBRP B. subtilis, Japan]
• BKE10560 ([gene|9118A36CD30E7C99697B3659BB04E03BB7EE71D7|ntdR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTTCCCTTCCTTAAA, downstream forward: _UP4_TAACGATAGATTTCCCTTTA
• BKK10560 ([gene|9118A36CD30E7C99697B3659BB04E03BB7EE71D7|ntdR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTTCCCTTCCTTAAA, downstream forward: _UP4_TAACGATAGATTTCCCTTTA

References

Reviews

• 21512256

Original publications

• 14612444,20817675,17056753