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ctsR [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]
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ctsR [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

transcription repressor of class III heat shock genes (clpC operon, clpE, clpP)
locus
BSU00830
pI
9.00
mw
17.00 kDa
function
regulation of protein degradation
product
transcription repressor
essential
no
synonyms
yacG

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [SW|Categories] containing this gene/protein

  • [SW|proteolysis], [SW|transcription factors and their control], [SW|general stress proteins (controlled by SigB)], [SW|heat shock proteins], [SW|phosphoproteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|CtsR regulon], [SW|SigB regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    101,449 -> 101,913

    The protein

    Protein family

  • ctsR family (according to Swiss-Prot)
  • Modification

  • phosphorylated on Arg-55 (phosphorylation serves as tag for degradation by [SW|ClpC]-[SW|ClpP]) [Pubmed|27749819]
  • phosphorylation of a tyrosine residue by [protein|search|McsB] [Pubmed|14984053]
  • recently, it was reported that CtsR is phosphorylated by [protein|search|McsB] on Arg-62 rather than on a tyrosine residue [Pubmed|19498169]
  • in addition, Arg-15 was reported to be a phosphorylation site [Pubmed|22517742]
  • Effectors of protein activity

  • CtsR is inactivated by heat, heat sensing requires Gly-64 [Pubmed|20852588]
  • non-phosphorylated [protein|search|McsB] targets CtsR for degradation [Pubmed|20852588]
  • regulated proteolysis by [protein|search|ClpP]/[protein|search|ClpC] [Pubmed|11179229,16163393,17380125]
  • Structure

  • [PDB|3H0D] (complex with a 26bp DNA duplex, from ''Geobacillus stearothermophilus'') [Pubmed|19498169]
  • [SW|Interactions]

  • [protein|search|McsA]-[protein|search|McsB]-[protein|search|CtsR]
  • [protein|search|CtsR]-[protein|search|ClpP]/[protein|search|ClpC] (degradation of [protein|search|CtsR]) [Pubmed|11179229,16163393,17380125]
  • Expression and Regulation

    Operon

  • ''[protein|search|ctsR]-[protein|search|mcsA]-[protein|search|mcsB]-[protein|search|clpC]-[protein|search|radA]-[protein|search|disA]'' [Pubmed|8793870]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|8793870], [protein|search|SigB] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2]
  • Regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|11544224]
  • repressed at low temperatures ([protein|search|CtsR]) [Pubmed|9987115]
  • Regulatory mechanism

  • [protein|search|CtsR]: transcription repression [Pubmed|9987115]
  • Additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
  • Biological materials

    Mutant

  • ''ctsR::aphA3'' availbale in [SW|Ulf Gerth]'s lab
  • BKE00830 (''[protein|search|ctsR]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab)
  • Expression vector

  • for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab
  • Antibody

  • available in [SW|Ulf Gerth]'s lab
  • References

    Reviews

  • 27518094
  • Original Publications

  • 14984053,21078442,23375660,27749819