ctsR

transcription repressor of class III heat shock genes ([gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] operon, [gene|8C5B14FE5E03427F9A598C75D4081FA0D6696299|clpE], [gene|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP])

locus: BSU_00830

pI: 9.26

mw: 17.60 kDa

protein length: 154 aa

gene length: 465 bp

function: regulation of protein degradation

product: transcription repressor

essential: no

synonyms: yacG

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 101,449 101,913

Phenotypes of a mutant

• reduced expression of [gene|1BC439BBCD5FE19D5780519D7E4317C2EFF0D21B|trxB], due to reduced [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx] activity [pubmed|30962353]

The protein

Protein family

• CtsR family (single member, according to UniProt)

Modification

• phosphorylated on Arg-55 (phosphorylation serves as tag for degradation by [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP]) [Pubmed|27749819]
• phosphorylation of a tyrosine residue by [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|McsB] [Pubmed|14984053]
• recently, it was reported that CtsR is phosphorylated by [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|McsB] on Arg-62 rather than on a tyrosine residue [Pubmed|19498169]
• in addition, Arg-15 was reported to be a phosphorylation site [Pubmed|22517742]

Effectors of protein activity

• CtsR is inactivated by heat, heat sensing requires Gly-64 [Pubmed|20852588]
• non-phosphorylated [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|McsB] targets CtsR for degradation [Pubmed|20852588]
• regulated proteolysis by [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP]/[protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC] [Pubmed|11179229,16163393,17380125]

Structure

• [PDB|3H0D] (complex with a 26bp DNA duplex, from ''Geobacillus stearothermophilus'') [Pubmed|19498169]
• [PDB|6FH4] (C-terminal domain bound to phosphoarginine) [pubmed|30962626]

additional information

• subject to Clp-dependent proteolysis upon glucose starvation [pubmed|17981983]
• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000017 PRODORIC2 database]

Expression and Regulation

Operons

• genes: [gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]-[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]-[gene|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]-[gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[gene|151F226370D225776F3FE7EA4901485095F1AC45|radA]-[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]
  description: [Pubmed|8793870]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|17434969], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8793870], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [pubmed|8793870] [pubmed|11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulatory mechanism

  • [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR]: repression, [pubmed|9987115,11179229,16163393,17380125], in [regulon|908DB17A39D518E84977250C55825E77FA02E391|CtsR regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [pubmed|30962353], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

  regulation

  • expressed during germination and spore outgrowth [Pubmed|24244006]
  • induction during diamide stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]) [pubmed|30962353]

additional information

• the mRNA is very stable (> 15 min) [pubmed|12884008]

Biological materials

Mutant

• GP3202([gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]::''ermC''), available in [SW|Jörg Stülke]'s lab
• MGNA-B928 (yacG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1927 NBRP B. subtilis, Japan]
• ''ctsR::aphA3'' availbale in [SW|Ulf Gerth]'s lab
• BKE00830 (''[gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
• BKE00830 ([gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCAACCCCCTCCTTTAC, downstream forward: _UP4_AAATTAAAATAAGCGGGTGA
• BKK00830 ([gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCAACCCCCTCCTTTAC, downstream forward: _UP4_AAATTAAAATAAGCGGGTGA

Expression vectors

• for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

Antibody

• available in [SW|Ulf Gerth]'s lab

References

Reviews

• 14984053,21078442,23375660,27518094,28402413,28748186

Original Publications

• 27749819,30962626,30962353,11179229,20852588