SubtiBank SubtiBank
parC
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

parC

subunit of DNA topoisomerase IV
locus
BSU18100
pI
6.31
mw
91.14 kDa
protein length
806 aa Sequence Blast
gene length
2418 bp Sequence Blast
function
chromosome segregation and compaction
product
subunit of DNA topoisomerase IV
essential
yes
ec
5.99.1.-
synonyms
grlA

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    1,935,448 → 1,937,868

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • a ''parC'' point mutation has been isolated upon selction at low pressure [Pubmed|26296725]
  • The protein

    Protein family

  • topoisomerase gyrA/parC subunit family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|B207C7328177924B16F1C606F667BEADF12CA069|GyrA]
  • Structure

  • [PDB|1WP5] (C-terminal domain, Geobacillus stearothermophilus)
  • [SW|Localization]

  • cytoplasm (Homogeneous) [Pubmed|16479537]
  • polar localization in actively growing cells [Pubmed|9539793]
  • Expression and Regulation

    Operons

    genes
    [gene|1901C52DCB24B3D53150F6926C14907AC1B75293|parE]-[gene|9075DD0CC0B8554F0379732453665F43C70EFC26|parC]
    description
    [Pubmed|16267290]

    regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]
  • regulation

  • induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]
  • additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''parE'' [Pubmed|27120414]
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    References

  • 16272394,15466871,16267290,16479537,9539793,26296725,12767818