cotX

spore crust protein (insoluble fraction), necessary for the assembly of the spore crust, the outermost layer of the spore coat

locus: BSU_11760

pI: 4.23

mw: 18.46 kDa

protein length: 172 aa

gene length: 519 bp

function: spore crust assembly

product: spore crust protein (insoluble fraction)

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.8|Not yet assigned]

Gene

Coordinates: 1,250,656 1,251,174

The protein

Modification

• contains a glycosylation motif [pubmed|31502725]

[SW|Localization]

• spore crust with a preference for spore poles [Pubmed|31502725,23202530]

Expression and Regulation

Operons

• genes: [gene|905148CAF82018E7F8E800FDF3F41163AAA8E682|cotX]
  description: [Pubmed|7519271]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, before [protein|63870866AA2EA1727D77609B330FCD9704007679|CotV] [Pubmed|26577401,15699190,7519271], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, before [protein|63870866AA2EA1727D77609B330FCD9704007679|CotV], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|7519271], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]
  • [protein|0D7F5504590512E1598B94DC1734BF8FF67ED994|GerR]: activation, [Pubmed|26577401], in [regulon|0D7F5504590512E1598B94DC1734BF8FF67ED994|GerR regulon]

  regulation

  • expressed late during sporulation ([SW|SigE], [protein|search|SigK], [protein|search|GerE], [protein|search|GerR]) [Pubmed|26577401]

• genes: [gene|63870866AA2EA1727D77609B330FCD9704007679|cotV]-[gene|47669BC8073FB0F9799E06BB9FC39700F960910E|cotW]-[gene|905148CAF82018E7F8E800FDF3F41163AAA8E682|cotX]
  description: [Pubmed|7519271]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,7519271], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15383836], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|7519271,15383836], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]
  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: activation, [Pubmed|15383836], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]

  regulation

  • expressed during sporulation in the mother cell ([protein|search|SigE], [protein|search|SigK], [protein|search|GerE], [SW|SpoIIID]) [Pubmed|15383836]

Biological materials

Mutant

• BKE11760 ([gene|905148CAF82018E7F8E800FDF3F41163AAA8E682|cotX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTATTCCTCCTTTTC, downstream forward: _UP4_TAGGACCTAAAAGCAGAGCT
• BKK11760 ([gene|905148CAF82018E7F8E800FDF3F41163AAA8E682|cotX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTATTCCTCCTTTTC, downstream forward: _UP4_TAGGACCTAAAAGCAGAGCT

References

Reviews

• 23202530,27227299

Original publications

• 7519271,10788508,20451384,12107147,21665972,21821766,26821119,26577401,30582883,31502725