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sdhB [Wed Aug 24 2016 13:22:41 GMT+0200 (CEST)]
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sdhB [Wed Aug 24 2016 13:22:41 GMT+0200 (CEST)]

succinate dehydrogenase
locus
BSU28430
pI
7.00
mw
28.00 kDa
function
TCA cycle
product
succinate dehydrogenase (iron-sulfur protein)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|membrane proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|FsrA regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,905,571 -> 2,906,332

    The protein

    Catalyzed reaction/ biological activity

  • Succinate acceptor = fumarate reduced acceptor (according to Swiss-Prot)

    • Protein family

  • succinate dehydrogenase/fumarate reductase iron-sulfur protein family (according to Swiss-Prot)

    • [SW|Cofactors]

  • [3 Fe- 4 S] cluster

    • Effectors of protein activity

  • Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide [Pubmed|3910107]
  • Activated by Cytochrome b558 [Pubmed|6799760]

    • Structure

  • [PDB|1NEK] (''E. coli'')

    • [SW|Localization]

  • attached to the membrane [Pubmed|18763711]

    • [SW|Interactions]

  • [protein|search|SdhA]-[protein|search|SdhB], [protein|search|SdhB]-[protein|search|SdhC] (as part of the [protein|search|SdhC]-[protein|search|SdhB]-[protein|search|SdhA] complex)
  • part of a [SW|respiratory supercomplex] [Pubmed|23880299]

    • Additional information

  • This enzyme is a trimer membrane-bound [Pubmed|3910107] [Pubmed|6799760]
  • One subunit is bound to citochrome b558, and this subunit is the one bound to the cytosolic side of the membrane [Pubmed|3910107] [Pubmed|6799760]
  • Another subunit is the flavoprotein one, required for FAD usage [Pubmed|3910107] [Pubmed|6799760]
  • The other subunit has an iron-sulphur domain necessary for the catalytic activity [Pubmed|3910107] [Pubmed|6799760]
  • extensive information on the structure and enzymatic properties of succinate dehydrogenase can be found at [http://www.proteopedia.org/wiki/index.php/Succinate_Dehydrogenase Proteopedia]

    • Expression and Regulation

    Operon

  • ''[protein|search|sdhC]''-''[protein|search|sdhA]''-''[protein|search|sdhB]'' [Pubmed|3036777]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|2495271]

    • Regulation

  • constitutive
  • part of the iron sparing response ([protein|search|FsrA]) [Pubmed|22389480]

    • Regulatory mechanism

  • [protein|search|FsrA]: translation inhibition [Pubmed|22389480]

    • Biological materials

    Mutant

  • GP792 (''[protein|search|sdhC]-[protein|search|sdhA]-[protein|search|sdhB]''::''phleo''), available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • GP1436 (spc, based on [SW|pGP1870]), available in the [SW|Stülke] lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] lab

    • FLAG-tag construct

  • GP1426 (spc, based on [SW|pGP1331]), available in the [SW|Stülke] lab

    • Antibody

  • **

    • References

    Reviews

  • 11803018

    • Original publications

  • 1707123,6401289,1324713,3036777,2495271,3027051,7748886,2837411,12560550,3910107,6799760,22389480,23880299