lspA

signal peptidase II

locus: BSU_15450

pI: 9.13

mw: 17.28 kDa

protein length: 154 aa

gene length: 465 bp

function: protein secretion

product: signal peptidase II

essential: no

ec: 3.4.23.36

synonyms: lsp

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,616,744 1,617,208

The protein

Catalyzed reaction/ biological activity

• Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains (according to UniProt)

Protein family

• peptidase A8 family (single member, according to UniProt)

Structure

• [PDB|5DIR] (from Pseudomonas aeruginosa, 38% identity), [pubmed|26912896]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|8FC1A0A6C9975F6C125490FBAAB2A4957210A9F1|lspA]-[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|ylyB]-[gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE15450 ([gene|8FC1A0A6C9975F6C125490FBAAB2A4957210A9F1|lspA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAACGTTCCTCCAGTTT, downstream forward: _UP4_GGGAAAAAGAAAAAGGAGCA
• BKK15450 ([gene|8FC1A0A6C9975F6C125490FBAAB2A4957210A9F1|lspA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAACGTTCCTCCAGTTT, downstream forward: _UP4_GGGAAAAAGAAAAAGGAGCA

References

• 9882689,10497172,9880550,26912896