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ispE [2019-07-19 10:32:18]
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ispE [2019-07-19 10:32:18]

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, fourth step in the MEP pathway of isoprenoid biosynthesis
locus
BSU_00460
pI
6.35
mw
31.57 kDa
protein length
289 aa Sequence Blast
gene length
870 bp Sequence Blast
function
MEP pathway of isoprenoid biosynthesis
product
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
essential
yes
ec
2.7.1.148
synonyms
yabH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    53,516 54,385

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol (according to UniProt)
  • 4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H+ (according to UniProt)

    • Protein family

  • GHMP kinase family (together with [protein|36930CC6FDFCA23D43E264FEA375B78A1434E1BD|GalK] and [protein|290302BA8A4E44C62AE3071C1F7DE18B20605607|ThrB]) (according to UniProt)

    • Structure

  • [PDB|1UEK] (from ''E. coli'', 38% identity) [Pubmed|12771135]

    • Biological materials

    Mutant

  • MGNA-B910 (yabH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1909 NBRP B. subtilis, Japan]

    • References

  • 17458547,23840410,12771135,29335298