SubtiBank SubtiBank
ispE
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ispE

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, fourth step in the MEP pathway of isoprenoid biosynthesis
locus
BSU00460
pI
6.35
mw
31.57 kDa
protein length
289 aa Sequence Blast
gene length
870 bp Sequence Blast
function
MEP pathway of isoprenoid biosynthesis
product
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
essential
yes
ec
2.7.1.148
synonyms
yabH

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    53,516 54,385

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (according to Swiss-Prot)
  • Protein family

  • GHMP kinase family (together with [protein|36930CC6FDFCA23D43E264FEA375B78A1434E1BD|GalK] and [protein|290302BA8A4E44C62AE3071C1F7DE18B20605607|ThrB]) (according to UniProt)
  • Structure

  • [PDB|1UEK] (from ''E. coli'', 38% identity) [Pubmed|12771135]
  • Expression and Regulation

    Operons

    genes
    [gene|8FA635D1915108BF29A864AF4DA28ECF633F735F|ispE]-[gene|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]-[gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR]: repression, [Pubmed|7638212], in [regulon|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR regulon]
  • regulation

  • negative autoregulation [Pubmed|7638212]
  • view in new tab

    Biological materials

    Mutant

  • MGNA-B910 (yabH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1909 NBRP B. subtilis, Japan]
  • References

  • 17458547,23840410,12771135,29335298