SubtiBank SubtiBank
ispE
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

ispE

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, fourth step in the MEP pathway of isoprenoid biosynthesis
locus
BSU00460
pI
6.35
mw
31.57 kDa
protein length
289 aa Sequence Blast
gene length
867 bp Sequence Blast
function
MEP pathway of isoprenoid biosynthesis
product
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
essential
yes
ec
2.7.1.148
synonyms
yabH

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    53,516 → 54,385

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (according to Swiss-Prot)
  • Protein family

  • IspE subfamily (according to Swiss-Prot)
  • Structure

  • [PDB|1UEK] (from ''E. coli'', 38% identity) [Pubmed|12771135]
  • Expression and Regulation

    Operons

    genes
    [gene|8FA635D1915108BF29A864AF4DA28ECF633F735F|ispE]-[gene|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]-[gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR]: repression, [Pubmed|7638212], in [regulon|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR regulon]
  • regulation

  • negative autoregulation [Pubmed|7638212]
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    Biological materials

    Mutant

  • MGNA-B910 (yabH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1909 NBRP B. subtilis, Japan]
  • References

  • 17458547,23840410,12771135