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plsX [2017-04-24 15:34:12]
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plsX [2017-04-24 15:34:12]

acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4), coordinates membrane synthesis with cell division
locus
BSU15890
pI
5.49
mw
35.61 kDa
protein length
333 aa Sequence Blast
gene length
999 bp Sequence Blast
function
biosynthesis of phospholipids
product
acyl-acyl carrier protein (ACP):phosphate acyltransferase
essential
yes
ec
2.3.1.n2
synonyms
ylpD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|ComA regulon], [SW|FapR regulon]

    • Gene

    Coordinates
    1,662,547 → 1,663,548

    Phenotypes of a mutant

  • essential [Pubmed|24224907,12682299]
  • depletion results in sporulation deficiency (via [protein|9F338225E1286D530EAAAB504DEC8D5AFA06AC8D|SpoIIR]) [Pubmed|22111992]
  • a ts mutant forms an abberant Z-ring and has a [SW|cell division] defect [Pubmed|24224907]

    • The protein

    Catalyzed reaction/ biological activity

  • catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)

    • Protein family

  • plsX family (according to Swiss-Prot)

    • Structure

  • [PDB|1VI1] [Pubmed|16021622]

    • [SW|Localization]

  • uniformly distributed on the membrane of most cells, but occasionally appears as membrane foci as well [Pubmed|26816052]
  • in the presence of daptomycin, PlsX delocalizes from the membrane to the cytoplasm [Pubmed|27791134]

    • Expression and Regulation

    Operons

    genes
    [gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]-[gene|8F5612395EBF90BEF4C80056EECAFC572384E7F8|plsX]-[gene|BB8542A6444564B21FF5DB6E2C75192C6DA2DFB8|fabD]-[gene|439B468A13137000FB42E9389391CB4986FFED84|fabG]-[gene|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|acpA]
    description
    [Pubmed|19850612]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16932747], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]: repression, [Pubmed|12737802], in [regulon|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR regulon]
  • [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]: activation, ([protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]) [Pubmed|16091051], in [regulon|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA regulon]

    • regulation

  • ''[protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]'': repressed in the absence of malonyl-CoA or malonyl-[protein|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|ACP] ([protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]) [Pubmed|12737802]
  • expression of the operon is strongly reduced during [SW|biofilm formation] [pubmed|31113899]
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    Biological materials

    two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in Matsumoto lab [Pubmed|19282621]

    • References

  • 12737802,19282621,17645809,17557823,8759840,16932747,19850612,16091051,21383089,22111992,24224907,26816052,16021622,27791134