SubtiBank SubtiBank
plsX
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plsX

acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)
Locus
BSU_15890
Isoelectric point
5.49
Molecular weight
35.61 kDa
Protein length
333 aa Sequence Blast
Gene length
1002 bp Sequence Blast
Function
biosynthesis of phospholipids
Product
acyl-acyl carrier protein (ACP):phosphate acyltransferase
Essential
yes
E.C.
2.3.1.n2
Synonyms
ylpD

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,662,547 1,663,548

Phenotypes of a mutant

  • essential PubMed
  • depletion results in sporulation deficiency (via SpoIIR) PubMed
  • a ts mutant forms an abberant Z-ring and has a cell division defect PubMed
  • depletion results in accumulation of malonyl-CoA and of long-chain-ACPs PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)
  • fatty acyl-[ACP] + phosphate --> acyl phosphate + holo-[ACP] (according to UniProt)
  • Protein family

  • plsX family (single member, according to UniProt)
  • Structure

    Localization

  • localizes to regions of increased fluidity in the membrane (via a short amphipathic α-peptide) PubMed
  • uniformly distributed on the membrane of most cells, but occasionally appears as membrane foci as well PubMed
  • in the presence of daptomycin, PlsX delocalizes from the membrane to the cytoplasm PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • FapR: repression, PubMed, in FapR regulon
  • ComA: activation, (ComA) PubMed, in ComA regulon
  • Regulation

  • FapR: repressed in the absence of malonyl-CoA or malonyl-ACP (FapR) PubMed
  • expression of the operon is strongly reduced during biofilm formation PubMed
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    Biological materials

    Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Matsumoto lab PubMed
  • References

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