plsX

acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO₄)

locus: BSU_15890

pI: 5.49

mw: 35.61 kDa

protein length: 333 aa

gene length: 1002 bp

function: biosynthesis of phospholipids

product: acyl-acyl carrier protein (ACP):phosphate acyltransferase

essential: yes

ec: 2.3.1.n2

synonyms: ylpD

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,662,547 1,663,548

Phenotypes of a mutant

• essential [Pubmed|24224907,12682299]
• depletion results in sporulation deficiency (via [protein|9F338225E1286D530EAAAB504DEC8D5AFA06AC8D|SpoIIR]) [Pubmed|22111992]
• a ts mutant forms an abberant Z-ring and has a [SW|cell division] defect [Pubmed|24224907]
• depletion results in accumulation of malonyl-CoA and of long-chain-ACPs [pubmed|32671874]

The protein

Catalyzed reaction/ biological activity

• catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO₄)
• fatty acyl-[ACP] + phosphate --> acyl phosphate + holo-[ACP] (according to UniProt)

Protein family

• plsX family (single member, according to UniProt)

Structure

• [PDB|1VI1] [Pubmed|16021622]
• [PDB|6A1K] [pubmed|31453380]

[SW|Localization]

• localizes to regions of increased fluidity in the membrane (via a short amphipathic α-peptide) [pubmed|31453380,31925177]
• uniformly distributed on the membrane of most cells, but occasionally appears as membrane foci as well [Pubmed|26816052]
• in the presence of daptomycin, PlsX delocalizes from the membrane to the cytoplasm [Pubmed|27791134]

Expression and Regulation

Operons

• genes: [gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]-[gene|8F5612395EBF90BEF4C80056EECAFC572384E7F8|plsX]-[gene|BB8542A6444564B21FF5DB6E2C75192C6DA2DFB8|fabD]-[gene|439B468A13137000FB42E9389391CB4986FFED84|fabG]-[gene|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|acpA]
  description: [Pubmed|19850612]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16932747], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]: repression, [Pubmed|12737802], in [regulon|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR regulon]
  • [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]: activation, ([protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]) [Pubmed|16091051], in [regulon|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA regulon]

  regulation

  • ''[protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]'': repressed in the absence of malonyl-CoA or malonyl-[protein|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|ACP] ([protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]) [Pubmed|12737802]
  • expression of the operon is strongly reduced during [SW|biofilm formation] [pubmed|31113899]

Biological materials

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in Matsumoto lab [Pubmed|19282621]

References

• 12737802,19282621,17645809,17557823,8759840,16932747,19850612,16091051,21383089,22111992,24224907,26816052,16021622,27791134,31453380,31925177,31919098,32180316,32671874