SubtiBank SubtiBank
groEL
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

groEL

chaperonin and co-repressor for [protein|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|HrcA]
locus
BSU06030
pI
4.53
mw
57.25 kDa
protein length
544 aa Sequence Blast
gene length
1632 bp Sequence Blast
function
protein folding and re-folding
product
chaperonin, co-repressor for [protein|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|HrcA]
essential
yes
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.2|Chaperones/ protein folding]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]
  • Gene

    Coordinates
    650,234 → 651,868

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • acts as co-repressor for [protein|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|HrcA]
  • Protein family

  • chaperonin (HSP60) family (according to Swiss-Prot)
  • Modification

  • phosphorylated on Arg-35, Arg-116, and Arg-282 [Pubmed|22517742]
  • phosphorylated on ser/ thr/ tyr [Pubmed|16493705], [Pubmed|17726680]
  • Structure

  • [PDB|1WE3] ([protein|8F3DEE8828CA6BAAAF6DB2DA4349EF7AA9DF89F8|GroEL]-[protein|0D8B3F1F86FDBAE0F3B3651F456340F147B394AF|GroES] complex from ''Thermus thermophilus'', 65% identity) [Pubmed|15296740]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • recruited to the membrane after ethanol stress [Pubmed|22268681]
  • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Expression and Regulation

    Operons

    genes
    [gene|0D8B3F1F86FDBAE0F3B3651F456340F147B394AF|groES]-[gene|8F3DEE8828CA6BAAAF6DB2DA4349EF7AA9DF89F8|groEL]
    description
    [Pubmed|1350777]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1350776], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|HrcA]: repression, [Pubmed|8576042], in [regulon|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|HrcA regulon]
  • regulation

  • induced by heat shock ([protein|search|HrcA]) [Pubmed|1350777,8576042]
  • view in new tab

    Biological materials

    Expression vector

  • for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP1000 available in [SW|Jörg Stülke]'s lab
  • for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP1002 available in [SW|Jörg Stülke]'s lab
  • References

    Reviews

  • 28271487
  • Original publications

  • 9303302,1350777,12082092,15378759,17726680,16493705,9303302,1350776,8576042,7592421,22517742,22268681,24263382,26458230,15296740,27184767