SubtiBank SubtiBank
rhiN
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

rhiN

galacturonyl hydrolase, catalyses intracellular degradation of disaccharides generated by YesX
Locus
BSU_07000
Isoelectric point
4.76
Molecular weight
38.52 kDa
Protein length
344 aa Sequence Blast
Gene length
1035 bp Sequence Blast
Function
intracellular degradation of disaccharides generated by YesX
Product
galacturonyl hydrolase, catalyses intracellular degradation of disaccharides generated by YesX
Essential
no
Synonyms
yesR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of pectin

    • Gene

    Coordinates
    764,781 765,815

    The protein

    Catalyzed reaction/ biological activity

  • 2-O-(4-deoxy-β-L-threo-hex-4-enopyranuronosyl)-α-L-rhamnopyranose + H2O --> 5-dehydro-4-deoxy-D-glucuronate + L-rhamnopyranose (according to UniProt)

    • Protein family

  • glycosyl hydrolase 105 family (with YteR, according to UniProt)

    • Structure

  • 3PMM (from Klebsiella pneumoniae, 30% identity)

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    rhiL-rhiF-rhiG-rhiN
    Description
    PubMed

    Regulation

  • induced in colonies growing on steamed soybeans PubMed
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B260 (yesR::erm), available at the NBRP B. subtilis, Japan
  • BKE07000 (rhiN::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTGTGCCATCTGCAACACTC, downstream forward: _UP4_TAAAGATAGCGGCGGGGGGA
  • BKK07000 (rhiN::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTGTGCCATCTGCAACACTC, downstream forward: _UP4_TAAAGATAGCGGCGGGGGGA

    • References

    Loading