buk

butyrate kinase

locus: BSU_24070

pI: 5.47

mw: 39.61 kDa

protein length: 363 aa

gene length: 1092 bp

function: utilization of branched-chain keto acids

product: butyrate kinase

essential: no

ec: 2.7.2.7

synonyms: yqiU,bkd

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]

Gene

Coordinates: 2,501,549 2,502,640

The protein

Catalyzed reaction/ biological activity

• ATP + butanoate --> ADP + butanoyl phosphate (according to UniProt)

Protein family

• acetokinase family (with [protein|DAA285C86692E8B47D48652E0973AE3FF091CBC3|AckA], according to UniProt)

Structure

• [PDB|1X9J] (from ''Thermotoga maritima'', 52% identity, 69% similarity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]-[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]-[gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]-[gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]-[gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]-[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]-[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB]
  description: [Pubmed|12823818]

  sigma factors

  • [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL]: sigma factor, [Pubmed|10094682], in [regulon|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL regulon]

  regulatory mechanism

  • [protein|4CEBD81F485DD0B660E297FFC34A0F5270652184|BkdR]: activation, [Pubmed|10094682], in [regulon|4CEBD81F485DD0B660E297FFC34A0F5270652184|BkdR regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|10094682], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]

Biological materials

Mutant

• MGNA-C378 (yqiU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2376 NBRP B. subtilis, Japan]
• BKE24070 ([gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATAACCTCCAGATCAA, downstream forward: _UP4_TAGACAGACAGGAGTGAGTC
• BKK24070 ([gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATAACCTCCAGATCAA, downstream forward: _UP4_TAGACAGACAGGAGTGAGTC

References

• 12427936,15241682,10094682,12823818