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ssbA [Thu Jan 21 2016 19:01:40 GMT+0100 (CET)]
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ssbA [Thu Jan 21 2016 19:01:40 GMT+0100 (CET)]

single-strand DNA-binding protein, part of the replisome, promoter of RecA DNA repair center assembly, required to internalize and to recombine ssDNA with homologous resident duplex
locus
BSU40900
pI
4.00
mw
18.00 kDa
function
DNA replication, DNA repair/ recombination
product
single-strand DNA-binding protein
essential
yes
synonyms
ssb

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]
  • [SW|Categories] containing this gene/protein

  • [SW|DNA replication], [SW|DNA repair/ recombination], [SW|essential genes], [SW|phosphoproteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|ComK regulon], [SW|stringent response]
  • Gene

    Coordinates on the chromosome (coding sequence)
    4,198,886 -> 4,199,404

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • SsbA inhibits ssDNA phosphorylase activity of [protein|search|PnpA] [Pubmed|21859751]
  • SsbA co-assembles onto [protein|search|SsbB]-coated ssDNA [Pubmed|22373918]
  • important for [protein|search|RecA] DNA repair center assembly by recruiting [protein|search|RecO] and [protein|search|RecR] to the [SW|replisome] [Pubmed|24891441]
  • RecA-ATP in concert with [protein|search|DprA] and [protein|search|SsbA] catalyzes DNA strand exchange, with [protein|search|SsbB] as an accessory factor [Pubmed|25138221]
  • Paralogous protein(s)

  • [protein|search|SsbB]
  • Modification

  • phosphorylated on Arg-76 [Pubmed|22517742]
  • phosphorylated on Tyr-82 by [protein|search|PtkA], dephosphorylated by [protein|search|PtpZ] [Pubmed|16549871]
  • Effectors of protein activity

  • Phosphorylation of Ssb increases binding to single-stranded DNA in vitro almost 200-fold [Pubmed|16549871]
  • Structure

  • [PDB|3VDY] (SsbB in complex with ssDNA, 63% identity, 87% similarity) [Pubmed|22373918]
  • [SW|Localization]

  • forms foci [Pubmed|21958350]
  • Nucleoid (Mid-cell) [Pubmed|16479537]
  • [SW|Interactions]

  • forms tetramers [Pubmed|22373918]
  • part of the [SW|replisome]: [protein|search|PolC]-[protein|search|HolA]-[protein|search|HolB]-[protein|search|DnaX]-[protein|search|DnaN]-[protein|search|DnaG]-[protein|search|DnaC]-[protein|search|DnaI]-[protein|search|DnaD]-[protein|search|SsbA]-[protein|search|DnaE]-[protein|search|PriA]-[protein|search|DnaB] [Pubmed|20122408]
  • [protein|search|SsbA]-[protein|search|SbcC] [Pubmed|21170359]
  • [protein|search|SsbA]-[protein|search|RecG] [Pubmed|21170359]
  • [protein|search|SsbA]-[protein|search|RecJ] [Pubmed|21170359]
  • [protein|search|SsbA]-[protein|search|YrvN] [Pubmed|21170359]
  • [protein|search|SsbA] (bound to ssDNA) -[protein|search|RecO], this helps to dislodge [protein|search|SsbA] and [protein|search|SsbB] from ssDNA [Pubmed|21170359,22373918]
  • [protein|search|SsbA]-[protein|search|YqiB] [Pubmed|21170359]
  • [protein|search|SsbA]-[protein|search|Ung] [Pubmed|21170359]
  • [protein|search|SsbA]-[protein|search|YpbB] [Pubmed|21170359]
  • [protein|search|SsbA]-[SW|RecD2] [Pubmed|24443534,21170359]
  • [protein|search|SsbA]-[protein|search|DnaN] [Pubmed|21958350]
  • [protein|search|SsbA] (bound to ssDNA) -[protein|search|RecO], this helps to dislodge [protein|search|SsbA] and [protein|search|SsbB] from ssDNA [Pubmed|21170359,22373918]
  • [protein|search|SsbA]-[protein|search|YocI] according to [Pubmed|22933559]
  • [protein|search|SsbA]-[protein|search|RecQ] according to [Pubmed|22933559]
  • Expression and Regulation

    Operon

  • ''[protein|search|yyaF]-[protein|search|rpsF]-[protein|search|ssbA]-[protein|search|rpsR]'' [Pubmed|14762004]
  • ''[protein|search|rpsF]-[protein|search|ssbA]-[protein|search|rpsR]'' [Pubmed|24310371,22383849]
  • [SW|Sigma factor]

  • ''[protein|search|rpsF]'': [protein|search|SigA] [Pubmed|24310371]
  • Regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • [protein|search|ComK]: transcription activation [Pubmed|11948146]
  • Biological materials

    two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab
  • References

    Reviews

  • 22976189,22933559
  • Original publications

  • 17853894,19730681,11948146,16479537,11948165,16549871,20122408,14762004,20581116,22054219,22373918,22517742,21859751,21170359,21958350,23268446,23536821,23779106,22383849,24310371,24443534,24891441,25138221,26707201