scpA [2018-05-09 12:56:35]

part of the [SW|condensin] complex, chromosomal origin condensation and segregation

locus: BSU23220

pI: 4.79

mw: 29.46 kDa

protein length: 251 aa

gene length: 753 bp

function: segregation of replication origins

product: DNA segregation and condensation protein

essential: yes

synonyms: ypuG

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,425,831 → 2,426,586

Phenotypes of a mutant

• essential [Pubmed|12682299]
• ''[gene|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth [Pubmed|24440399,24440393]

The protein

Protein family

• scpA family (according to Swiss-Prot)

Structure

• [PDB|3ZGX] (complex between the head domain of [protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|Smc] (aa 1 - 219 and 983 - 1186) and the N-terminal winged-helix domain of [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|ScpA]) [Pubmed|23353789]
• [PDB|4I98] (complex between [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|ScpA] (aa 1-160)-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|ScpB] (aa 1-183) [Pubmed|23353789]

[SW|Localization]

• nucleoid (Multiple) [Pubmed|16479537]
• the [SW|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|Smc])2-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|ScpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|ScpB] complex forms two bipolar assemblies on the chromosome, one in each cell half, localization depends on [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|ParA] [Pubmed|24440393,23475963]*the [SW|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|Smc])2-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|ScpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|ScpB] complex is loaded by [protein|EF4BD49FCD49EE97908973581478951C8FA196C0|ParB] to ''parS'' centromeric sites adjacent to the replication origin, travels then from the origin to the terminus at rates >50 kb per minute [Pubmed|28154080,24440393,23475963]

Expression and Regulation

Operons

• genes: [gene|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]-[gene|3CC628BCF8F9F45E598060B775008B361DB2127A|ypuI]
  description: [Pubmed|7934830]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

  regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

Biological materials

Mutant

• MGNA-A104 (ypuG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/104 NBRP B. subtilis, Japan]

References

Reviews

• 22934648,22933559,24118085,25460803,26706151,29182912

Original publications

• 12100548,12421306,12065423,7934830,16479537,19450516,15009890,11948165,23353789,23475963,22385855,24440399,24440393,25557547,26253537,25951515,26295962,26725510,26904953,28154080,28286005,28689660