SubtiBank SubtiBank
pgpH [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]
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pgpH [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

c-di-AMP specific phosphodiesterase
locus
BSU25330
pI
7.00
mw
78.00 kDa
function
control of c-di-AMP homeostasis
product
c-di-AMP specific phosphodiesterase
essential
no
synonyms
yqfF

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.3|Targets of (p)ppGpp]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|metabolism of signalling nucleotides], [SW|membrane proteins]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,612,282 -> 2,614,417

    Phenotypes of a mutant

  • a ''[SW|gdpP] [SW|pgpH]'' double mutant acquires suppressor mutations in ''[SW|cdaA]'' [Pubmed|26240071]
  • a ''[SW|gdpP] [SW|pgpH]'' double mutant is defective in [SW|biofilm formation] [Pubmed|27252699]
  • The protein

    Catalyzed reaction/ biological activity

  • hydrolysis of c-di-AMP to 5'-pApA [Pubmed|25583510]
  • [SW|Domains]

  • [SW|HD domain]
  • [SW|Cofactors]

  • Mn2 [Pubmed|25583510]
  • Effectors of protein activity

  • ppGpp acts as allosteric inhibitor of phosphodiesterase activity (in ''Listeria monocytogenes'') [Pubmed|25583510]
  • Structure

  • [PDB|4S1B] (the HD domain of the protein from ''Listeria monocytogenes'' in complex with c-di-AMP, 58% identity, 85% similarity) [Pubmed|25583510]
  • [SW|Localization]

  • cell membrane [Pubmed|25583510]
  • [SW|Interactions]

  • [protein|search|PgpH] is a member of a suspected group of hubs proteins that were suggested to be involved in a large number of [SW|interactions] [Pubmed|21630458]
  • Biological materials

    Mutant

  • SM-GN1 (''pgpH-spc''), available in [SW|Anne Galinier]'s and [SW|Boris Görke]'s labs
  • BKE25330 (''[protein|search|pgpH]''::''erm'' without terminator, available in the BGSC and in [SW|Jörg Stülke]'s lab)
  • GP2033 (''[protein|search|pgpH]''::''tet'', available in [SW|Jörg Stülke]'s lab)
  • GP2034 (''[protein|search|pgpH]''::''erm'' without terminator, available in [SW|Jörg Stülke]'s lab) [Pubmed|26240071]
  • GP2040 (''[protein|search|gdpP]''::''spc'' ''[protein|search|pgpH]''::''erm'' without terminator, available in [SW|Jörg Stülke]'s lab) [Pubmed|26240071]
  • GP2049 (''[protein|search|pgpH]''::''cat'' without terminator, available in [SW|Jörg Stülke]'s lab)
  • lacZ fusion

  • pGP190 (in [protein|search|pAC7]), available in [SW|Stülke] lab
  • References

    Reviews

  • 25637595,25869574,26773214
  • Original publications

  • 21630458,25583510,26240071,27252699