alaS [Fri Apr 01 2016 16:24:48 GMT+0200 (CEST)]

alanine-tRNA synthetase

locus: BSU27410

pI: 4.00

mw: 97.00 kDa

function: translation

product: alanine-tRNA synthetase

essential: yes

ec: 6.1.1.7

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

[SW|Categories] containing this gene/protein

• [SW|translation], [SW|essential genes]

This gene is a member of the following [SW|regulons]

• [SW|T-box]

Gene

Coordinates on the chromosome (coding sequence): 2,798,174 -> 2,800,810

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP L-alanine tRNA(Ala) = AMP diphosphate L-alanyl-tRNA(Ala) (according to Swiss-Prot)

Protein family

• class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

Structure

• [PDB|1YFS] (enzyme from ''Aquifex aeolicus'') [Pubmed|15657145], [PDB|3HXU] (enzyme from ''E. coli'' with alanine) [Pubmed|20010690]

Expression and Regulation

Regulation

• induced by alanine limitation ([protein|search|T-box]) [Pubmed|19258532]

Regulatory mechanism

• [protein|search|T-box]: [SW|RNA switch], transcriptional antitermination [Pubmed|19258532]

References

• 19258532,15657145,20010690,26426057