SubtiBank SubtiBank
alaS
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

alaS

alanine-tRNA synthetase
locus
BSU27410
pI
4.92
mw
97.08 kDa
protein length
878 aa Sequence Blast
gene length
2634 bp Sequence Blast
function
translation
product
alanine-tRNA synthetase
essential
yes
ec
6.1.1.7
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,798,174 → 2,800,810

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP L-alanine tRNA(Ala) = AMP diphosphate L-alanyl-tRNA(Ala) (according to Swiss-Prot)
  • Protein family

  • class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Structure

  • [PDB|1YFS] (enzyme from ''Aquifex aeolicus'') [Pubmed|15657145]
  • [PDB|3HXU] (enzyme from ''E. coli'' with alanine) [Pubmed|20010690]
  • Expression and Regulation

    Operons

    genes
    [gene|8C5C7F9A585AE89D53EAF1DB3A9AB525B9E30B4A|alaS]-[gene|C5457FEC46A1AFC6E5B1148B0E5F258B2C3197B2|yrzL]-[gene|508FC7117B4CE7AF119B3830AE9DC113CB058039|yrrK]-[gene|A0AA5289F5E92A4B2E18E4F3439F2D412208826E|yrzB]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [regulon|T-box|T-box]: antitermination, in [regulon|T-box|T-box]
  • regulation

  • induced by alanine limitation ([regulon|T-box|T-box]) [Pubmed|19258532]
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    References

  • 19258532,15657145,20010690,26426057