brnQ

branched-chain amino acid transporter

locus: BSU_26690

pI: 9.78

mw: 46.84 kDa

protein length: 440 aa

gene length: 1323 bp

function: uptake of valine and isoleucine

product: branched-chain amino acid transporter

essential: no

synonyms: yrdJ

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.5|BCAA transporters]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,727,160 2,728,482

Phenotypes of a mutant

• no phenotype for the single mutant, the triple ''[gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP] [gene|8BB8BDA602CC26B0A9E8C22666F0EC539306CF39|brnQ] [gene|75A874F0C344E8404BE39C32EFF59CE562193640|braB]'' mutant is strongly impaired in the transport of isoleucine and valine at low concentrations [Pubmed|25645558]

The protein

Catalyzed reaction/ biological activity

• high affinity uptake of isoleucine and valine [Pubmed|25645558]

Protein family

• branched chain amino acid transporter family (together with [protein|75A874F0C344E8404BE39C32EFF59CE562193640|BraB]) (according to UniProt)

Paralogous protein(s)

• [protein|75A874F0C344E8404BE39C32EFF59CE562193640|BraB]

Expression and Regulation

Operons

• genes: [gene|D7397591C2157EBB1951ED3DC1E315F9AE7A4BB3|azlB]-[gene|C90D642197EE589F9D33CD5AA0B1069E3BC124A8|azlC]-[gene|7A1445B7AFD9C3AA45EF67E51F5D233FCC5F8884|azlD]-[gene|8BB8BDA602CC26B0A9E8C22666F0EC539306CF39|brnQ]-[gene|17FFEA4977F4F7334CFF7CD1C644DF14F599E6E6|yrdK]
  description: [Pubmed|9287000]

  regulatory mechanism

  • [protein|D7397591C2157EBB1951ED3DC1E315F9AE7A4BB3|AzlB]: repression, [Pubmed|9287000], in [regulon|D7397591C2157EBB1951ED3DC1E315F9AE7A4BB3|AzlB regulon]

  regulation

  • repressed by [protein|search|AzlB] [Pubmed|9287000]

Biological materials

Mutant

• BKE26690 ([gene|8BB8BDA602CC26B0A9E8C22666F0EC539306CF39|brnQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE26690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATATCCTCCGATTA, downstream forward: _UP4_ATTCCAGCTATTGCAGGAGG
• BKK26690 ([gene|8BB8BDA602CC26B0A9E8C22666F0EC539306CF39|brnQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK26690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATATCCTCCGATTA, downstream forward: _UP4_ATTCCAGCTATTGCAGGAGG

References

• 9287000,25645558