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ylnF
probably precorrin-2 dehydrogenase
function
siroheme biosynthesis , sulfite reduction
product
probably precorrin-2 dehydrogenase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.6|Biosynthesis of heme/ siroheme]Gene
Coordinates
1,635,603 1,636,091
The protein
Catalyzed reaction/ biological activity
NAD+ + precorrin-2 --> 2 H+ + NADH + sirohydrochlorin (according to UniProt)Protein family
ferrochelatase family (with [protein|E4C588EC93132CCBE4F40559F8A480A66A2D82D7|HemH], according to UniProt)Structure
[PDB|3DFZ] (36% identity) [pubmed|18588505]Biological materials
Mutant
MGNA-B370 (ylnF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1369 NBRP B. subtilis, Japan]BKE15630 ([gene|8BAB9B12D9D58A45446DC6CB7F48869089800FD3|ylnF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCACAACCTTCTTCTTTT, downstream forward: _UP4_TAAGCCAGAGCATACAAATGBKK15630 ([gene|8BAB9B12D9D58A45446DC6CB7F48869089800FD3|ylnF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCACAACCTTCTTCTTTT, downstream forward: _UP4_TAAGCCAGAGCATACAAATGReferences
10217486,16267287,11004190,12107147,18039762,18588505