yxdL

[SW|ABC transporter] (ATP-binding protein)

locus: BSU_39640

pI: 7.36

mw: 28.57 kDa

protein length: 257 aa

gene length: 774 bp

function: export of toxic peptides

product: [SW|ABC transporter] (ATP-binding protein)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.3|Export of peptides]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 4,070,393 4,071,166

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|8BF7EB539B53D75BD30BD30ABF27106D6A0B030B|FtsE], [protein|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|BceA], [protein|FE3A01BEA974C5E32C68DC600C800E5EF101B34F|YknY], [protein|4FE6A51A0D9BBBF574EA2EF18B95B5BFC0A64CCB|YtrE], [protein|0F8048267EC038D8CF673317D969BA27735473A7|YvrO], [protein|464DD1855E6B3195EE242637612A16C73FF32FC0|PsdA]

[SW|Domains]

• [SW|ABC transporter domain] (aa 5-243) (according to UniProt)

Structure

• [PDB|1L2T] (from Methanocaldococcus jannaschii, 43% identity) [pubmed|12150914]

[SW|Localization]

• plasma membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|8B4267532B9788AEB5EF39E2695C1FDA494C7055|yxdL]-[gene|4423383A3F6DAFDFA210F4BEEF7D8CBEC674DCEA|yxdM]-[gene|6D9153BA698101C3EE1CD68B76419ECD300740F8|yxeA]
  description: [Pubmed|15289557]

  regulatory mechanism

  • [protein|F3C5505E311E6DDABAFA87899F28CF6596CDBA63|YxdJ]: activation, [Pubmed|15289557], in [regulon|F3C5505E311E6DDABAFA87899F28CF6596CDBA63|YxdJ regulon]

  regulation

  • induced by a cationic antimicrobial peptide, LL-37 ([protein|search|YxdJ]) [Pubmed| 21078927]

Biological materials

Mutant

• MGNA-B704 (yxdL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1703 NBRP B. subtilis, Japan]
• BKE39640 ([gene|8B4267532B9788AEB5EF39E2695C1FDA494C7055|yxdL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAAATCCTCCTTATGG, downstream forward: _UP4_CTGTCTATGCTGGGGGGAAA
• BKK39640 ([gene|8B4267532B9788AEB5EF39E2695C1FDA494C7055|yxdL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAAATCCTCCTTATGG, downstream forward: _UP4_CTGTCTATGCTGGGGGGAAA

References

• 17600057,10092453,15289557,21283517