SubtiBank SubtiBank
serA [Mon May 16 2016 15:55:35 GMT+0200 (CEST)]
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serA [Mon May 16 2016 15:55:35 GMT+0200 (CEST)]

phosphoglycerate dehydrogenase
locus
BSU23070
pI
5.00
mw
56.00 kDa
function
biosynthesis of serine
product
phosphoglycerate dehydrogenase
essential
no
ec
1.1.1.95
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.8|Biosynthesis/ acquisition of serine/ glycine/ alanine]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis/ acquisition of amino acids], [SW|membrane proteins], [SW|most abundant proteins]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,411,086 -> 2,412,663

    The protein

    Catalyzed reaction/ biological activity

  • 3-phospho-D-glycerate NAD = 3-phosphonooxypyruvate NADH (according to Swiss-Prot)
  • Protein family

  • D-isomer specific 2-hydroxyacid dehydrogenase family (according to Swiss-Prot)
  • Modification

  • S-cysteinylation after diamide stress (C410)[Pubmed|17611193]
  • active site Cys410 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species [Pubmed|21749987] [Pubmed|22938038]
  • Structure

  • [PDB|1YBA] (from ''E. coli'', 30% identity, 52% similarity) [Pubmed|15823035]
  • [SW|Localization]

  • membrane [Pubmed|18763711]
  • Expression and Regulation

    Operon

  • ''serA'' [Pubmed|7934830]
  • Regulation

  • strongly repressed in response to glucose starvation in M9 medium [Pubmed|23033921]
  • Additional information

  • belongs to the 100 [[most abundant proteins]] [PubMed|15378759]
  • Biological materials

    Mutant

  • 1A614 ( ''serA''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A614&Search=1A614 BGSC] and in [SW|Jörg Stülke]'s lab
  • lacZ fusion

  • pGP187 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab
  • References

  • 22938038,17611193,7934830,18763711,15823035,21749987,23033921,15378759