serA [Mon May 16 2016 15:55:35 GMT+0200 (CEST)]

phosphoglycerate dehydrogenase

locus: BSU23070

pI: 5.00

mw: 56.00 kDa

function: biosynthesis of serine

product: phosphoglycerate dehydrogenase

essential: no

ec: 1.1.1.95

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.8|Biosynthesis/ acquisition of serine/ glycine/ alanine]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

[SW|Categories] containing this gene/protein

• [SW|biosynthesis/ acquisition of amino acids], [SW|membrane proteins], [SW|most abundant proteins]

Gene

Coordinates on the chromosome (coding sequence): 2,411,086 -> 2,412,663

The protein

Catalyzed reaction/ biological activity

• 3-phospho-D-glycerate NAD = 3-phosphonooxypyruvate NADH (according to Swiss-Prot)

Protein family

• D-isomer specific 2-hydroxyacid dehydrogenase family (according to Swiss-Prot)

Modification

• S-cysteinylation after diamide stress (C410)[Pubmed|17611193]
• active site Cys410 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species [Pubmed|21749987] [Pubmed|22938038]

Structure

• [PDB|1YBA] (from ''E. coli'', 30% identity, 52% similarity) [Pubmed|15823035]

[SW|Localization]

• membrane [Pubmed|18763711]

Expression and Regulation

Operon

• ''serA'' [Pubmed|7934830]

Regulation

• strongly repressed in response to glucose starvation in M9 medium [Pubmed|23033921]

Additional information

• belongs to the 100 [[most abundant proteins]] [PubMed|15378759]

Biological materials

Mutant

• 1A614 ( ''serA''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A614&Search=1A614 BGSC] and in [SW|Jörg Stülke]'s lab

lacZ fusion

• pGP187 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab

References

• 22938038,17611193,7934830,18763711,15823035,21749987,23033921,15378759