bsdB

phenolic acid decarboxylase

Locus: BSU_03630

Isoelectric point: 10.06

Molecular weight: 22.39 kDa

Protein length: 204 aa

Gene length: 615 bp

Function: resistance to salicylic acid

Product: phenolic acid decarboxylase

Essential: no

Synonyms: yclB

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Coping with stress → Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

Gene

Coordinates: 412,540 413,154

The protein

Catalyzed reaction/ biological activity

• conversion of vanillic acid to guaiacol PubMed
• dimethylallyl phosphate + FMNH₂ --> phosphate + prenyl-FMNH₂ (according to UniProt)

Protein family

• UbiX/PAD1 family (single member, according to UniProt)

Structure

• 1SBZ (from E. coli, 57% identity) PubMed

Expression and Regulation

Operons

• Genes: bsdB-bsdC-bsdD
  Description: PubMed

  Regulatory mechanism

  • BsdA: activation, PubMed, in BsdA regulon

  Regulation

  • induced by salicylic acid (BsdA) PubMed

Biological materials

Mutant

• MGNA-C061 (yclB::erm), available at the NBRP B. subtilis, Japan
• BKE03630 (bsdB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCAATCATACTCCTGA, downstream forward: _UP4_AAACAAAAAGGAGGAGCTTG
• BKK03630 (bsdB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCAATCATACTCCTGA, downstream forward: _UP4_AAACAAAAAGGAGGAGCTTG

References

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