SubtiBank SubtiBank
nusA [Mon May 16 2016 17:53:47 GMT+0200 (CEST)]
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nusA [Mon May 16 2016 17:53:47 GMT+0200 (CEST)]

transcription termination factor of RNA polymerase
locus
BSU16600
pI
4.00
mw
41.00 kDa
function
transcription
product
transcription termination factor
essential
yes
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.3|Transcription elongation/ termination]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [SW|Categories] containing this gene/protein

  • [SW|transcription], [SW|essential genes]
  • This gene is a member of the following [SW|regulons]

  • [SW|stringent response]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,732,281 -> 1,733,396

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • stimulates [SW|transcription] termination at weak intrinsic terminators [http://www.nature.com/articles/nmicrobiol20157 Reference]
  • Protein family

  • nusA family (according to Swiss-Prot)
  • Structure

  • [PDB|1HH2] (from ''Thermotoga maritima'', 51% identity) [Pubmed|11430821]
  • [SW|Interactions]

  • [protein|search|NusA]-[protein|search|RpoB] [Pubmed|19680289]
  • binds elongating [SW|RNA polymerase] [http://www.nature.com/articles/nmicrobiol20157 Reference]
  • Expression and Regulation

    Operon

  • ''[protein|search|ylxS]-[protein|search|nusA]-[protein|search|ylxR]-[protein|search|ylxQ]-[protein|search|infB]-[protein|search|ylxP]-[protein|search|rbfA]'' [Pubmed|8491709]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|8491709]
  • Regulation

  • autoregulation [SW|NusA] [http://www.nature.com/articles/nmicrobiol20157 reference]
  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • Regulatory mechanism

  • [SW|NusA]: [SW|transcription] attenuation, [SW|NusA] stimulates termination [http://www.nature.com/articles/nmicrobiol20157 Reference]
  • Additional information

  • there are two [SW|NusA]-stimulated terminators in the untranslated region upstream of ''[SW|NusA]'', they allow maintenance of [SW|NusA] homeostasis [http://www.nature.com/articles/nmicrobiol20157 Reference]
  • Biological materials

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab
  • References

    Reviews

  • 26132790
  • Original publications

  • not in PubMed: [http://www.nature.com/articles/nmicrobiol20157 Mondal et al., 2016, Nature Microbiology]
  • 11948165,8491709,19680289,12161562,10027981,15978071,20817769,11430821