SubtiBank SubtiBank
trmD
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

trmD

tRNA methyltransferase, methylates G37 residue at the N1 position in a subset of tRNAs
locus
BSU16030
pI
5.31
mw
27.56 kDa
protein length
243 aa Sequence Blast
gene length
729 bp Sequence Blast
function
tRNA modification
product
tRNA methyltransferase
essential
yes
ec
2.1.1.31
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    1,675,171 → 1,675,902

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine (according to Swiss-Prot)
  • Protein family

  • RNA methyltransferase trmD family (according to Swiss-Prot)
  • Structure

  • [PDB|3KY7] (from ''Staphylococcus aureus'', 62% identity)
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)