trmD

tRNA methyltransferase, methylates G37 residue at the N1 position in a subset of tRNAs

locus: BSU_16030

pI: 5.31

mw: 27.56 kDa

protein length: 243 aa

gene length: 732 bp

function: tRNA modification

product: tRNA methyltransferase

essential: yes

ec: 2.1.1.31

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 1,675,171 1,675,902

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• guanosine³⁷ in tRNA + S-adenosyl-L-methionine --> H⁺ + N¹-methylguanosine³⁷ in tRNA + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• RNA methyltransferase trmD family (single member, according to UniProt)

Structure

• [PDB|3KY7] (from ''Staphylococcus aureus'', 62% identity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|8F559BFC80FE56EA0389A2336F4E9E6908562BCB|ylxM]-[gene|EC025F78D0BCBD91162BAAB9EAB7D3837E0A3208|ffh]-[gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP]-[gene|67FB5F6FB0B2701BD8E4FDD8DF06B236DE6A2599|ylqC]-[gene|0AB1FB44E8F28944B82B5EF14A9CDCFEE46DB76B|ylqD]-[gene|34B0FA6E7E25D1E5D8A54B70F5562BD0BD05B498|rimM]-[gene|885ECD9FAAC8BC95D32F049825D6B6244C046112|trmD]-[gene|51368512B8322EDBCA92674EC2B97CF26087D8A9|rplS]
  description: [Pubmed|29794222]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8335643], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • the mRNA is processed between [gene|EC025F78D0BCBD91162BAAB9EAB7D3837E0A3208|ffh] and [gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]