SubtiBank SubtiBank
tagE

tagE

UDP-glucose:polyglycerol phosphate glucosyltransferase
locus
BSU_35730
pI
6.03
mw
78.12 kDa
protein length
673 aa Sequence Blast
gene length
2022 bp Sequence Blast
function
biosynthesis of teichoic acid
product
UDP-glucose:polyglycerol phosphate glucosyltransferase
essential
no
ec
2.4.1.52
synonyms
rodD,gtaA,gtaD

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.4|Biosynthesis of teichoic acid]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.3|Biosynthesis of teichoic acid]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • Gene

    Coordinates
    3,678,399 3,680,420

    Phenotypes of a mutant

  • inactivation of ''tagE'' is advantageous in environments with fluctuating glucose availability [Pubmed|23924781]
  • resistant to phage SPO1 [pubmed|30811056]
  • The protein

    Catalyzed reaction/ biological activity

  • transfer of glucose from UDP-glucose to glycerol phosphate in polymeric form [Pubmed|21558268]
  • 4-O-{[(2R)-1-glycerylphospho](n)-(2R)-1-glycerylphospho}-N-acetyl-β-D-mannosaminyl-(1→4)-N-acetyl-α-D-glucosaminyl undecaprenyl diphosphate + n UDP-α-D-glucose --> 4-O-{[(2R)-2-α-D-glucosyl-1-glycerylphospho](n)-(2R)-1-glycerylphospho}-N-acetyl-β-D-mannosaminyl-(1→4)-N-acetyl-α-D-glucosaminyl undecaprenyl diphosphate + n H+ + n UDP (according to UniProt)
  • Protein family

  • [SW|glycosyltransferase 1 family] (according to UniProt)
  • [SW|Glycosyltransferase 4 subfamily] (according to UniProt)
  • Modification

  • phosphorylation on Ser-2 [Pubmed|17218307]
  • Structure

  • [PDB|4X7R] (from Staphylococcus aureus, 26% identity) [pubmed|25624472]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|8620DA76A1953D7ACDFA7E46ED81EF1A0D97999C|tagD]-[gene|88028A5F3520CB6DAB014412974EB59CC5D226DD|tagE]-[gene|E730E41546298960A4B907FCB7F36C495AA9602B|tagF]-[gene|6795F1703416DCD04E51C177F1C9FE45E73ABAEF|tagG]-[gene|4D3C316965834130BB378CBD1265CFA13F086C7A|tagH]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7581998], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: repression, [Pubmed|9457886], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]: activation, [Pubmed|12950927], in [regulon|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR regulon]
  • regulation

  • repressed by phosphate ([protein|search|PhoP]) [Pubmed|9457886]
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    Biological materials

    Mutant

  • 1A981 ( ''tagE''::''erm''), [Pubmed|18487323], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A981&Search=1A981 BGSC]
  • BKE35730 ([gene|88028A5F3520CB6DAB014412974EB59CC5D226DD|tagE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTTTACTCCCTTTCG, downstream forward: _UP4_GTCACGGAAATAAAAGAGAG
  • BKK35730 ([gene|88028A5F3520CB6DAB014412974EB59CC5D226DD|tagE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTTTACTCCCTTTCG, downstream forward: _UP4_GTCACGGAAATAAAAGAGAG
  • References

    Reviews

  • 24024634
  • Original publications

  • 9457886,7581998,2507871,17218307,12950927,23924781,21558268,22383849,25624472,30811056