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gltC [Mon Aug 31 2015 13:54:39 GMT+0200 (CEST)]
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gltC [Mon Aug 31 2015 13:54:39 GMT+0200 (CEST)]

Transcriptional activator of the gltA-gltB operon. Activates expression of the operon in the absence of arginine.
33.90 kDa
positive regulation
of the glutamate synthase operon (gltAB)
transcriptional regulator (LysR family)

Genomic Context



  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW|Transcription factors/ other]
  • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis/ acquisition of amino acids], [SW|glutamate metabolism], [SW|transcription factors and their control]
  • Gene

    Phenotypes of a mutant

  • ''gltC'' mutants are auxotrophic for glutamate.
  • The protein

    Catalyzed reaction/ biological activity

  • transcription activation of the ''[protein|search|gltA]-[protein|search|gltB]'' operon [Pubmed|2548995]
  • Protein family

  • [SW|LysR family] [Pubmed|2548995]
  • Paralogous protein(s)

  • none, but there are 19 members of the LysR family in ''B. subtilis
  • [SW|Domains]

  • DNA-binding helix-turn-helix motif: AA 18 ... 37
  • Effectors of protein activity

  • 2-oxoglutarate stimulates transcription activation, glutamate inhibits transcription activation [Pubmed|17134717]
  • [SW|Interactions]

  • active as dimer
  • [protein|search|GltC]-[protein|search|RocG], This interaction takes place in the presence of glutamate. It prevents the transcription activation of the ''[protein|search|gltA]-[protein|search|gltB]'' operon. Note that [protein|search|RocG] expression is strongly regulated by carbon and nitrogen sources, respectively [Pubmed|17608797]
  • [protein|search|GltC]-[protein|search|GudB] [Pubmed|25711804]
  • Expression and Regulation


  • ''[protein|search|gltC]'' (according to [ DBTBS])
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|2548995]
  • Regulation

  • autoregulation by GltC [Pubmed|2548995]
  • Regulatory mechanism

  • autorepression [Pubmed|2548995]
  • Biological materials


  • GP344 (erm), (available in [SW|Jörg Stülke]'s lab)
  • GP738 (spc), (available in [SW|Jörg Stülke]'s lab)
  • GP1904 (''gltC''::''aphA3''), (available in [SW|Jörg Stülke]'s lab)
  • Expression vector

  • for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP903, available in [SW|Jörg Stülke]'s lab
  • for expression, purification in ''E. coli'' with C-terminal Strep-tag, in pET3C: pGP951, available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • Antibody

  • available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [ Homepage]
  • [SW|Jörg Stülke], University of Göttingen, Germany
  • [ Homepage]
  • [SW|Fabian Commichau] University of Göttingen, Germany
  • [ Homepage]
  • References


  • 20408793,22625175
  • Original Publications

  • 7559360,15150225,2548995,17183217,17608797,17134717,14523131,20630473,25711804