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gltC [2017-02-27 15:02:04]
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gltC [2017-02-27 15:02:04]

Transcriptional activator of the gltA-gltB operon. Activates expression of the operon in the absence of arginine.
33.90 kDa
protein length
300 aa Sequence Blast
gene length
900 bp Sequence Blast
positive regulation
of the glutamate synthase operon (gltAB)
transcriptional regulator (LysR family)

Genomic Context



  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW|Transcription factors/ other]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,014,779 -> 2,015,681

    Phenotypes of a mutant

  • ''gltC'' mutants are auxotrophic for glutamate.
  • The protein

    Catalyzed reaction/ biological activity

  • transcription activation of the ''[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]'' operon [Pubmed|2548995]
  • Protein family

  • [SW|LysR family] [Pubmed|2548995]
  • Paralogous protein(s)

  • none, but there are 19 members of the LysR family in ''B. subtilis
  • [SW|Domains]

  • DNA-binding helix-turn-helix motif: AA 18 ... 37
  • Effectors of protein activity

  • 2-oxoglutarate stimulates transcription activation, glutamate inhibits transcription activation [Pubmed|17134717]
  • Expression and Regulation

    Biological materials


  • GP344 (erm), (available in [SW|Jörg Stülke]'s lab)
  • GP738 (spc), (available in [SW|Jörg Stülke]'s lab)
  • GP1904 (''gltC''::''aphA3''), (available in [SW|Jörg Stülke]'s lab)
  • Expression vector

  • for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP903, available in [SW|Jörg Stülke]'s lab
  • for expression, purification in ''E. coli'' with C-terminal Strep-tag, in pET3C: pGP951, available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • Antibody

  • available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [ Homepage]
  • [SW|Jörg Stülke], University of Göttingen, Germany
  • [ Homepage]
  • [SW|Fabian Commichau] University of Göttingen, Germany
  • [ Homepage]
  • References


  • 20408793,22625175
  • Original Publications

  • 7559360,15150225,2548995,17183217,17608797,17134717,14523131,20630473,25711804