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dltC [2019-04-16 12:51:42]
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dltC [2019-04-16 12:51:42]

D-alanine carrier protein, alanylation of teichoic acid provides some resistance against positively charged antimicrobial peptides
locus
BSU38520
pI
3.62
mw
8.87 kDa
protein length
78 aa Sequence Blast
gene length
237 bp Sequence Blast
function
biosynthesis of teichoic acid
product
D-alanine carrier protein
essential
no
ec
6.1.1.13
synonyms
ipa-3r
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.4|Biosynthesis of teichoic acid]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.3|Biosynthesis of teichoic acid]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

    • Gene

    Coordinates
    3,954,987 3,955,223

    Phenotypes of a mutant

  • more sensitive to nisin [Pubmed|23980836]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP + D-alanine + poly(ribitol phosphate) = AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate) (according to Swiss-Prot)

    • Modification

  • modified with the 4-phosphopantetheine (Ppant) group at Ser35, by [protein|200BE4802FCE3C860565B374EAADC9C1FEEF3E49|AcpS] [pubmed|30283133], then further modified with a d-alanyl group by [protein|795CD64B65D7CBF26E5F706C7617196CC6FCF864|DltA], through a thioester bond [pubmed|30283133]

    • Structure

  • [PDB|4BPG] [Pubmed|26193422]

    • Expression and Regulation

    Operons

    genes
    [gene|459ED28A98F4EC7E8A9016C742DC8EB5C26B5E65|ywzH]-[gene|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA]-[gene|1B9BAEBE029BE9D4AD7E49C38B1E6DB5E8F4D4D3|dltB]-[gene|862D977CF1E185597329A1EF0915485F72482A03|dltC]-[gene|D178EC8A1FF22C99C437E01FC4843C5176564992|dltD]-[gene|2FB3F09DA60A40212FE20217A64DAE714AB807CF|dltE]
    description
    [Pubmed|29794222]

    sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|7797557], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]
  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, [Pubmed|14762009], in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]
  • [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV]: sigma factor, [Pubmed|21926231], in [regulon|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV regulon]

    • regulatory mechanism

  • [protein|7098319D8E88FDC2A629A3F4A21507FD7A649385|YvrHb]: activation, [Pubmed|16306698], in [regulon|7098319D8E88FDC2A629A3F4A21507FD7A649385|YvrHb regulon]
  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|7797557], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

    • regulation

  • expression is reduced in a [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV] mutant [Pubmed|21926231]
  • the mRNA is processed between [gene|459ED28A98F4EC7E8A9016C742DC8EB5C26B5E65|ywzH] and [gene|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
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    Biological materials

    Mutant

  • BKE38520 ([gene|862D977CF1E185597329A1EF0915485F72482A03|dltC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAAATCCATAGCTTTCTAAT, downstream forward: _UP4_AATCAGCTGTCTGAGTTGAA
  • BKK38520 ([gene|862D977CF1E185597329A1EF0915485F72482A03|dltC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAAATCCATAGCTTTCTAAT, downstream forward: _UP4_AATCAGCTGTCTGAGTTGAA

    • References

    Reviews

  • 24819367

    • Original publications

  • 14762009,15955059,26193422,7797557,23980836,21856855,21926231,11434765,30283133