transcriptional pleiotropic regulator (MerR family) involved in global nitrogen regulation
function
regulation of nitrogen assimilation
product
transcription activator/ repressor (MerR family)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.5|Regulators of core metabolism][SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of amino acids], [SW|glutamate metabolism], [SW|transcription factors and their control], [SW|regulators of core metabolism]This gene is a member of the following [SW|regulons]
[SW|GlnR regulon], [SW|TnrA regulon]Gene
Coordinates on the chromosome (coding sequence)
1,397,411 -> 1,397,743
The protein
Protein family
[SW|MerR family]Effectors of protein activity
feedback-inhibited [protein|search|GlnA] prevents [protein|search|TnrA] from DNA bindingStructure
[PDB|4R22] (the TnrA-DNA complex) [Pubmed|25691471][http://pdb.org/pdb/search/structidSearch.do?structureId=4s0r 4S0R] (the [protein|search|TnrA]-[protein|search|GlnA] complex) [Pubmed|25691471][SW|Localization]
membrane-associated via [protein|search|NrgA]-[protein|search|NrgB] under conditions of poor nitrogen supply [Pubmed|21435182][SW|Interactions]
[protein|search|TnrA]-[protein|search|NrgB] [Pubmed|17001076][protein|search|TnrA]-[protein|search|GlnA], this interaction results in loss of [protein|search|TnrA] DNA-binding activity as well as in inhibition of [protein|search|GlnA] [Pubmed|23535029,11719184]Expression and Regulation
Operon
''tnrA'' (according to [http://dbtbs.hgc.jp/COG/prom/tnrA.html DBTBS])Regulation
expression is autocativated (TnrA) and repressed by [protein|search|GlnR] [Pubmed|16547045]expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|10671441,16547045]Regulatory mechanism
[protein|search|TnrA]: transcription activation [PubMed|10671441,16547045]Biological materials
Mutant
GP252 (in frame deletion), available in the [SW|Stülke] labBKE13310 (''tnrA''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab)1A851 ( ''tnrA''::''erm''), [Pubmed|8799114], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A851&Search=1A851 BGSC]1A851 ( ''tnrA''::''erm''), [Pubmed|8799114], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A851&Search=1A851 BGSC]Expression vector
for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP171 available in [SW|Stülke] labpGP229 (N-terminal Strep-tag, for [SW|SPINE], expression in ''B. subtilis'', in [SW|pGP380]), available in [SW|Stülke] labAntibody
available in the [SW|Karl Forchhammer] labLabs working on this gene/protein
[SW|Susan Fisher], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage][SW|Maria Schumacher], Durham, NC, USA [http://www.dukeschumacherlab.com/index.html homepage]References
Reviews
22625175,10231480,18086213 The [SW|TnrA regulon]
Control of TnrA activity by the [SW|trigger enzymes|trigger enzyme] [protein|search|GlnA]
11719184,12139611,17085574,19233925,16885465,26635369 Other original publications
12374841,9287005,12950915,10671441,16547045,8799114,15150225,11029411,17001076,15547269,2573733,8636055,16493705,6141156,18667567,21435182,23535029,23808168,25157083,25691471[Pubmed|] 
