SubtiBank SubtiBank
obg [Mon Jan 18 2016 18:32:16 GMT+0100 (CET)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

obg [Mon Jan 18 2016 18:32:16 GMT+0100 (CET)]

GTP-binding protein involved in initiation of sporulation and assembly of the 50S ribosome subunit
locus
BSU27920
pI
4.00
mw
47.00 kDa
function
ribosome assembly, may be required to stimulate activity
product
GTP-binding protein
essential
Yes
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.3|GTP-binding proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|translation], [SW|phosphorelay], [SW|essential genes], [SW|GTP-binding proteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,852,661 -> 2,853,947

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • Binds and hydrolyzes GTP and readily exchanges GDP for GTP

    • Protein family

  • GTP1/OBG family (according to Swiss-Prot) Era/Obg family

    • Paralogous protein(s)

  • [protein|search|Era], [protein|search|Obg], [protein|search|YphC], [protein|search|YsxC], [protein|search|RbgA], [protein|search|YqeH], [protein|search|ThdF], [protein|search|YyaF], [protein|search|YnbA]

    • [SW|Domains]

  • Obg possesses 3 domains: A N-terminal glycine-rich domain (1–158), a GTP binding domain (159–342) and a C-terminal TGS domain (343–428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.

    • [SW|Cofactors]

  • GDP, GTP and probably ppGpp

    • Structure

  • [PDB|1LMZ], [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=1LNZ 1LNZ]

    • [SW|Interactions]

  • [protein|search|RplM]-[protein|search|Obg] [Pubmed|10781545,20830302]
  • Interaction with Rsb proteins.

    • Additional information

  • The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in ''B. subtilis''.

    • Expression and Regulation

    Operon

  • ''[SW|spo0B]-[protein|search|obg]-[protein|search|yszB]-[protein|search|pheA]'' [Pubmed|22383849]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|2537815]

    • Biological materials

    Expression vector

  • for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP835, available in [SW|Stülke] lab
  • for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP843, available in [SW|Stülke] lab

    • Labs working on this gene/protein

  • [SW|Bill Haldenwang], San Antonio, USA
  • [SW|Naotake Ogasawara], Nara, Japan

    • References

    Reviews

  • 19575570,21885683

    • Original publications

  • 15325267,18689482,7961487,7961486,15583165,2537815,10781545,10419966,12427945,12429099,2537815,20830302