SubtiBank SubtiBank
proS
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

proS

prolyl-tRNA synthetase
locus
BSU16570
pI
5.01
mw
63.13 kDa
protein length
564 aa Sequence Blast
gene length
1692 bp Sequence Blast
function
translation
product
prolyl-tRNA synthetase
essential
yes
ec
6.1.1.15
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    1,725,330 → 1,727,024

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro) (according to Swiss-Prot)
  • Protein family

  • ProS type 1 subfamily (according to Swiss-Prot)
  • Structure

  • [PDB|2I4L] (from ''Rhodopseudomonas palustris'', 51% identity, 71% similarity) [Pubmed|17027500]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|58CDF08A8F05C0BC25EB6E400316F85FD0F307E5|ispC]-[gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP]-[gene|848570563D3C92A8E1E6AB4DA530388D8AE94E75|proS]-[gene|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC]
    description
    [Pubmed|22383849]
    view in new tab

    References

  • 17027500