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dnaN [Tue Jan 03 2017 17:30:25 GMT+0100 (CET)]
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dnaN [Tue Jan 03 2017 17:30:25 GMT+0100 (CET)]

DNA polymerase III (beta subunit), beta clamp, part of the replisome, also involved in DNA mismatch repair, inhibitor of DnaA oligomerization
locus
BSU00020
pI
4.00
mw
41.00 kDa
function
DNA replication, DNA repair
product
DNA polymerase III
(beta subunit), beta clamp
essential
yes
ec
2.7.7.7
synonyms
dnaG

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.7|Mismatch repair (MMR)]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [SW|Categories] containing this gene/protein

  • [SW|DNA replication], [SW|essential genes]
  • This gene is a member of the following [SW|regulons]

  • [SW|Spo0A regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,939 -> 3,075

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • [protein|search|DnaN] overexpression or release from the [SW|replisome] decreases association of [protein|search|YabA] with ''oriC'', increases association of [protein|search|DnaA] with ''oriC'' [Pubmed|21895792]
  • Deoxynucleoside triphosphate DNA(n) = diphosphate DNA(n 1) (according to Swiss-Prot)
  • recruits [protein|search|MutS] to the site of [SW|DNA replication] ([SW|replisome]) [Pubmed|23228104]
  • required for bacteriophage SPP1 replication [Pubmed|23268446]
  • inhibits oligomerization and helix formation of [protein|search|DnaA] [Pubmed|23909787]
  • Structure

  • [PDB|2AWA] (from ''Streptococcus pneumoniae'', 41% identity, 77% similarity)
  • [SW|Localization]

  • nucleoid (mid-cell spot) [Pubmed|9822387] [Pubmed|16479537]
  • forms large assemblies on DNA, called clamp zones [Pubmed|21419346]
  • forms foci [Pubmed|21958350]
  • [SW|Interactions]

  • [protein|search|DnaA]-[protein|search|YabA]-[protein|search|DnaN] [Pubmed|12060778]
  • part of the [SW|replisome]: [protein|search|PolC]-[protein|search|HolA]-[protein|search|HolB]-[protein|search|DnaX]-[protein|search|DnaN]-[protein|search|DnaG]-[protein|search|DnaC]-[protein|search|DnaI]-[protein|search|DnaD]-[protein|search|SsbA]-[protein|search|DnaE]-[protein|search|PriA]-[protein|search|DnaB] [Pubmed|20122408]
  • [SW|PolY1]-[protein|search|DnaN], [SW|PolY2]-[protein|search|DnaN] (required for untargeted mutagenesis) [Pubmed|15469515]
  • [SW|PolY1]-[protein|search|PolA]-[protein|search|DnaN] [Pubmed|16045613]
  • [protein|search|MutL] (C-terminal domain)-[protein|search|DnaN], the interaction stimulates the endonuclease activity of [SW|MutL] [Pubmed|26384423,21050827]
  • [protein|search|MutS]-[protein|search|DnaN] [Pubmed|20453097]
  • [protein|search|DnaE]-[protein|search|DnaN] [Pubmed|21958350]
  • [protein|search|DnaN] forms dimers [Pubmed|16045613]
  • Expression and Regulation

    Operon

  • ''[protein|search|dnaA]-[protein|search|dnaN]'' [Pubmed|2987848]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|2987848]
  • Regulation

  • negatively controlled by [protein|search|DnaA] [Pubmed|2168872] and [SW|Spo0A] [Pubmed|14651647]
  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
  • Regulatory mechanism

  • [SW|Spo0A]: transcription repression [Pubmed|14651647]
  • Biological materials

    GFP fusion

  • [Pubmed|16942601] [Pubmed|9822387]
  • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab
  • Labs working on this gene/protein

  • [SW|Philippe Noirot], Jouy-en-Josas, France [http://locus.jouy.inra.fr/cms/index.php?id=18 homepage]
  • References

    Reviews

  • 20157337,22933559,26343983
  • Original publications

  • 16942601,9822387,14651647,2987848,2168872,14651647,12060778,16461910,16479537,19737352,19081080,11395445,20122408,20451384,15469515,16045613,21895792,20453097,23228104,23268446,21050827,21419346,21958350,23909787,25170813,26384423,26615189,27369079