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dnaN [2017-10-05 11:37:59]
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dnaN [2017-10-05 11:37:59]

DNA polymerase III (beta subunit), beta clamp, part of the replisome, also involved in DNA mismatch repair, inhibitor of DnaA oligomerization
locus
BSU00020
pI
4.72
mw
41.94 kDa
protein length
378 aa Sequence Blast
gene length
1134 bp Sequence Blast
function
DNA replication, DNA repair
product
DNA polymerase III (beta subunit), beta clamp
essential
yes
ec
2.7.7.7
synonyms
dnaG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.7|Mismatch repair (MMR)]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    1,939 → 3,075

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • [protein|83E05071DEC874248D3E96B8F3A093C65939B314|DnaN] overexpression or release from the [SW|replisome] decreases association of [protein|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|YabA] with ''oriC'', increases association of [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] with ''oriC'' [Pubmed|21895792]
  • Deoxynucleoside triphosphate DNA(n) = diphosphate DNA(n 1) (according to Swiss-Prot)
  • recruits [protein|E2A8B04CD729332F9E6A13195181983F751F2F25|MutS] to the site of [SW|DNA replication] ([SW|replisome]) [Pubmed|23228104]
  • required for bacteriophage SPP1 replication [Pubmed|23268446]
  • inhibits oligomerization and helix formation of [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] [Pubmed|23909787]

    • Structure

  • [PDB|2AWA] (from ''Streptococcus pneumoniae'', 41% identity, 77% similarity)

    • [SW|Localization]

  • nucleoid (mid-cell spot) [Pubmed|9822387] [Pubmed|16479537]
  • forms large assemblies on DNA, called clamp zones [Pubmed|21419346]
  • forms foci [Pubmed|21958350]

    • Expression and Regulation

    Operons

    genes
    [gene|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]-[gene|83E05071DEC874248D3E96B8F3A093C65939B314|dnaN]
    description
    [Pubmed|2987848]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2987848], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

    • regulation

  • negatively controlled by [protein|search|DnaA] [Pubmed|2168872] and [SW|Spo0A] [Pubmed|14651647]

    • additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''dnaA'' [Pubmed|27120414]
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    Biological materials

    GFP fusion

  • [Pubmed|16942601] [Pubmed|9822387]

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

    • Labs working on this gene/protein

  • [SW|Philippe Noirot], Jouy-en-Josas, France [http://locus.jouy.inra.fr/cms/index.php?id=18 homepage]

    • References

    Reviews

  • 20157337,22933559,26343983,28075389

    • Original publications

  • 16942601,9822387,14651647,2987848,2168872,14651647,12060778,16461910,16479537,19737352,19081080,11395445,20122408,20451384,15469515,16045613,21895792,20453097,23228104,23268446,21050827,21419346,21958350,23909787,25170813,26384423,26615189,27369079