SubtiBank SubtiBank
dnaN
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

dnaN

DNA polymerase III (beta subunit), beta clamp, part of the [SW|replisome], also involved in DNA mismatch repair, inhibitor of [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] oligomerization
locus
BSU00020
pI
4.72
mw
41.94 kDa
protein length
378 aa Sequence Blast
gene length
1134 bp Sequence Blast
function
[category|SW 3.1.1|DNA replication], DNA repair
product
DNA polymerase III (beta subunit), beta clamp
essential
yes
ec
2.7.7.7
synonyms
dnaG

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    1,939 → 3,075

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • [protein|83E05071DEC874248D3E96B8F3A093C65939B314|DnaN] overexpression or release from the [SW|replisome] decreases association of [protein|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|YabA] with ''oriC'', increases association of [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] with ''oriC'' [Pubmed|21895792]
  • Deoxynucleoside triphosphate DNA(n) = diphosphate DNA(n 1) (according to Swiss-Prot)
  • recruits [protein|E2A8B04CD729332F9E6A13195181983F751F2F25|MutS] to the site of [SW|DNA replication] ([SW|replisome]) [Pubmed|23228104]
  • required for bacteriophage SPP1 replication [Pubmed|23268446]
  • inhibits oligomerization and helix formation of [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] [Pubmed|23909787]
  • Structure

  • [PDB|2AWA] (from ''Streptococcus pneumoniae'', 41% identity, 77% similarity)
  • [SW|Localization]

  • nucleoid (mid-cell spot) [Pubmed|9822387] [Pubmed|16479537]
  • forms large assemblies on DNA, called clamp zones [Pubmed|21419346]
  • forms foci [Pubmed|21958350]
  • Expression and Regulation

    Operons

    genes
    [gene|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]-[gene|83E05071DEC874248D3E96B8F3A093C65939B314|dnaN]
    description
    [Pubmed|2987848]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2987848], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • regulation

  • negatively controlled by [protein|search|DnaA] [Pubmed|2168872] and [SW|Spo0A] [Pubmed|14651647]
  • additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''dnaA'' [Pubmed|27120414]
  • view in new tab

    Biological materials

    GFP fusion

  • [Pubmed|16942601] [Pubmed|9822387]
  • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab
  • Labs working on this gene/protein

  • [SW|Philippe Noirot], Jouy-en-Josas, France [http://locus.jouy.inra.fr/cms/index.php?id=18 homepage]
  • References

    Reviews

  • 20157337,22933559,26343983,28075389
  • Original publications

  • 16942601,9822387,14651647,2987848,2168872,14651647,12060778,16461910,16479537,19737352,19081080,11395445,20122408,20451384,15469515,16045613,21895792,20453097,23228104,23268446,21050827,21419346,21958350,23909787,25170813,26384423,26615189,27369079,28878042