SubtiBank SubtiBank
argS
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

argS

arginyl-tRNA synthetase, universally conserved protein
locus
BSU37330
pI
5.11
mw
62.55 kDa
protein length
556 aa Sequence Blast
gene length
1668 bp Sequence Blast
function
translation
product
arginyl-tRNA synthetase
essential
yes
ec
6.1.1.19
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]
  • Gene

    Coordinates
    3,833,650 → 3,835,320

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg) (according to Swiss-Prot)
  • Protein family

  • class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Structure

  • [PDB|3FNR] (from ''Campylobacter jejuni'', 39% identity, 56% similarity)
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Additional information

  • [SW|universally conserved protein]
  • Expression and Regulation

    Operons

    genes
    [gene|83721D87607FE6448C2C0D17E401C87C57A05AF8|argS]
    description
    [Pubmed|9353933]
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    genes
    [gene|D71F54220FCC2B687857F53F53584D6CB90650CD|ywiB]-[gene|83721D87607FE6448C2C0D17E401C87C57A05AF8|argS]
    description
    [Pubmed|22383849]
    view in new tab

    additional information

  • the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
  • References

  • 9353933,26883633