SubtiBank SubtiBank
ywaA [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]
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ywaA [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]

branched-chain amino acid aminotransferase
locus
BSU38550
pI
4.00
mw
40.00 kDa
function
biosynthesis of branched-chain amino acids
product
branched-chain amino acid aminotransferase
essential
no
ec
2.6.1.42
synonyms
ipa-0r

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids]
  • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis/ acquisition of amino acids], [SW|most abundant proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|CodY regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    3,957,391 -> 3,958,482

    The protein

    Catalyzed reaction/ biological activity

  • L-leucine 2-oxoglutarate = 4-methyl-2-oxopentanoate L-glutamate (according to Swiss-Prot)
  • Protein family

  • class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|search|YbgE]
  • Modification

  • S-cysteinylation after diamide stress (C104) [Pubmed|17611193]
  • Structure

  • [PDB|3HT5] (from '' Mycobacterium tuberculosis'', 42% identity, 58% similarity) [Pubmed|19923721]
  • Expression and Regulation

    Operon

  • ''ywaA'' [Pubmed|9353933]
  • Regulation

  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|24163341]
  • Regulatory mechanism

  • [protein|search|CodY]: transcription repression [Pubmed|24163341]
  • Additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Biological materials

    Mutant

  • a ''ywaA::spc'' mutant and a ''[protein|search|bcd] [protein|search|ybgE] [protein|search|ywaA]'' triple mutant are available in [SW|Linc Sonenshein]'s lab
  • References

  • 12670965,17611193,24163341,15378759