mediator of RecA binding to ssDNA, required for the formation of RecA DNA repair centers, required for efficient survival and replication restart after replication-transcription conflicts
function
DNA repair/ recombination
product
mediator of RecA binding to ssDNA
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins][SW|Categories] containing this gene/protein
[SW|DNA repair/ recombination]Gene
Coordinates on the chromosome (coding sequence)
2,608,946 -> 2,609,713
Phenotypes of a mutant
drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) [Pubmed|24285298]''[protein|search|recO] [protein|search|recJ]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]''[protein|search|recO] [protein|search|dprA]'' double mutants have a strongly reduced chromosomal transformation rate [Pubmed|23779106,22373918]''[protein|search|recO] [protein|search|addA]-[protein|search|addB]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]The protein
Catalyzed reaction/ biological activity
provides [protein|search|RecA] access to ssDNA during chromosomal transformation (together with [protein|search|DprA]) [Pubmed|22373918]catalyzes annealing of [protein|search|SsbA] or [protein|search|SsbA]/[protein|search|SsbB] coated ssDNAs to allow the formation of DNA duplexes with tails during plasmid transformation [Pubmed|22373918]required for the formation of [protein|search|RecA] DNA repair centers (together with [protein|search|RecR]) [Pubmed|24891441]Protein family
recO family (according to Swiss-Prot)Structure
[PDB|2V1C] (the [protein|search|RecR]-[protein|search|RecO] complex from ''Deinococcus radiodurans'', [protein|search|RecR]: 52% identity, 78% similarity, [protein|search|RecO]: 30%/ 58%) [Pubmed|17581636][SW|Localization]
cytoplasm (according to Swiss-Prot)localizes to the DNA entry pole during transformation [Pubmed|22373918][SW|Interactions]
[protein|search|SsbA] (bound to ssDNA) -[protein|search|RecO], this helps to dislodge [protein|search|SsbA] and [protein|search|SsbB] from ssDNA [Pubmed|21170359,22373918][protein|search|RecR]-[protein|search|RecO], [protein|search|RecR]:[protein|search|RecO] molecular ratio of 2:1 (as judged from other bacteria such as ''E. coli'' and ''Deinococcus radiodurans'') [Pubmed|17581636]Biological materials
Mutant
1A892 ( ''recO''::''cat''), [Pubmed|10323239], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A892&Search=1A892 BGSC]References
Reviews
Original publications
24891441,18599486,19730681,20581116,22373918,17581636,21170359,24285298,24285298,25939832,26001966,15186413,10323239,26786319,23779106 
