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walR [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]
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walR [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]

two-component response regulator (OmpR family), controls cell wall metabolism
locus
BSU40410
pI
4.00
mw
27.00 kDa
function
control of cell wall metabolism
product
two-component response regulator (OmpR family)
essential
yes
synonyms
yycF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

    • [SW|Categories] containing this gene/protein

  • [SW|cell wall/ other], [SW|transcription factors and their control], [SW|essential genes], [SW|phosphoproteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    4,153,696 -> 4,154,403

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • a constitutively active form of [protein|search|WalR] ([protein|search|WalR]-R204C) results in constitutive expression of the [protein|search|WalR] regulon, and in combination with a ''[protein|search|sepF]'' mutation to the formation of cell wall-less L-forms [Pubmed|22122227]

    • The protein

    Protein family

  • [SW|OmpR family] of two-component response regulators

    • Modification

  • phosphorylated by [protein|search|WalK] on an Asp residue
  • phosphorylated by [protein|search|PrkC] on Thr-101, this stimulates [protein|search|WalR] activity [Pubmed|26102633]

    • Effectors of protein activity

  • phosphorylation likely affects DNA-binding activity

    • Structure

  • [PDB|2D1V] (DNA binding domain), [PDB|2ZWM] (receiver domain)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • [SW|Interactions]

  • [protein|search|WalK]-[protein|search|WalR]

    • Expression and Regulation

    Operon

  • ''[protein|search|walR]-[protein|search|walK]-[protein|search|yycH]-[protein|search|yycI]-[protein|search|walJ]-[protein|search|htrC]'' [Pubmed|9829949]

    • Regulation

  • expressed during vegetative growth, repressed during stationary phase [Pubmed|9829949]
  • induced at high temperature [Pubmed|24125693]

    • Biological materials

    Expression vector

  • pGP2633, constitutive expression in ''B. subtilis'' (based on [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP2635, expression of His-[protein|search|WalR] in ''E. coli'' (based on [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Kevin Devine], Dublin

    • References

    Reviews

  • 19019149,18245295,18792692,22126136,10094672

    • The [SW|WalR regulon]

  • 17581128,10878122,12950927,23199363

    • Other original Publications

  • 18573169,17350627,10094672,17827301,9829949,16030236,20111065,20167622,20834167,24125693,22122227,24125693,24490131,18789936,26102633